Evaluation of Biofield Treatment on Physical, Atomic and Structural Characteristics of Manganese (II, III) Oxide

Journal: Material Science & Engineering Received May 25, 2015; Accepted June 23, 2015; Published July 03, 2015

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Material Science & Engineering
Received May 25, 2015; Accepted June 23, 2015; Published July 03, 2015


Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O (2015) Evaluation of Biofield Treatment on Physical, Atomic and Structural Characteristics of Manganese (II, III) Oxide. J Material Sci Eng 4: 177. doi: 10.4172/2169- 0022.1000177


Evaluation of Biofield Treatment on Physical, Atomic and Structural Characteristics of Manganese (II, III) Oxide

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Trivedi MK, Nayak G, Patil S*, Tallapragada RM and Latiyal O

DOI: 10.4172/2169-0022.1000177   ISSN: 2169-0022


Trivedi MK, Nayak G, Patil S, Tallapragada RM, Latiyal O (2015) Evaluation of Biofield Treatment on Physical, Atomic and Structural Characteristics of Manganese (II, III) Oxide. J Material Sci Eng 4: 177. doi: 10.4172/2169- 0022.1000177


In Mn3O4, the crystal structure, dislocation density, particle size and spin of the electrons plays crucial role in modulating its magnetic properties. Present study investigates impact of Biofield treatment on physical and atomic properties of Mn3O4. X-ray diffraction revealed the significant effect of biofield on lattice parameter, unit cell volume, molecular weight, crystallite sizes and densities of treated Mn3O4. XRD analysis confirmed that crystallinity was enhanced and dislocation density was effectively reduced by 80%. FTIR spectroscopic analysis revealed that Mn-O bond strength was significantly altered by biofield treatment. Electronic spin resonance analysis showed higher g-factor of electron in treated Mn3O4 as compared to control, along with altered spin-spin atomic interaction of Mn with other mixed valance states. Additionally, ESR study affirmed higher magnetization behaviour of the treated Mn3O4. The results demonstrated that treated Mn3O4 ceramic could be used as an excellent material for fabrication of novel magnetic data storage devices.

Keywords: Biofield treatment, Mn3O4, X-ray diffraction, FT-IR, Paramagnetic, ESR, Brunauer-Emmett-Teller analysis, Particle size analysis.


Transition metal oxides (TMOs) constitute most interesting classes of solids, which exhibits different varieties of structures and properties [1]. Manganese (II, III) oxides (Mn3O4) is an excellent example of TMOs which gained significant attention among researchers due to its wide range of applications in magnetic materials, catalysis, ion exchange, magnetic data storage, super capacitors, molecular adsorption and ferrite materials [2-8]. Mn3O4 shows a paramagnetic behaviour at room temperature and ferromagnetic below 41-43K. The magnetic properties of Mn3O4 strongly depend on dislocations, vacancies, crystallite sizes, and lattice parameters. This affirms that crystal structure and its properties play an exclusive role in controlling magnetic strength in Mn3O4 that can be exploited in magnetic data storage applications. Mn3O4 exists as normal spinal crystal structure, in which Mn+2occupy a tetrahedral position and Mn+3 at octahedral positions [3-4].

Recently, magnetism and electrochemical properties in Mn3O4 nanoparticles are controlled by modulating the crystal structure by various processes such as annealing at high temperature [9], doping [10], hydrothermal [11], ultrasonic bath [12] and co-precipitation etc. Physical and chemical properties like particle size, surface area of Mn3O4 nanoparticles are controlled by various methods including vapor phase growth [13], thermal decomposition, chemical liquid precipitation and solvothermal [14-15].

Nevertheless each technique has their own advantages but there are certain drawbacks which limit their applicability at commercial level, such as vapour deposition method required high pressure and temperature to produce highly crystalline powder whereas thermal decomposition method requires specialized surfactants which may cause impurities in the product [16]. It has been already reported that magnetic behaviour can be improved by increasing the crystallinity [9] and particle size volume [16]. Hence in order to develop highly crystalline Mn3O4 nanoparticles and to improve its applicability at commercial level a simple and cost effective method should be designed.Biofield treatment is an excellent and cost effective approach which was recently used to modulate the, atomic structure [17-18]and density [19-21] molecular weight [22-23]of the bound atom thereby it facilitates the conversion of energy into mass and vice versa.Mr Trivedi is known for utilizing his biofield,referred herein as biofield treatment, for conducting experiments in various sectors such as material science [17-24], agriculture [25-29]and microbiology[30-32], which are already reported elsewhere.Biofield treatment had significantly changed the physical, atomic and thermal properties in transition metals [17,18, 20], carbon allotropes [19]and metal oxide ceramics [21,23]such as particle size was decreased by 71% in zirconium oxide [23] and crystallite size was increased by 66% in Vanadium Pentoxide(V2O5) [21]. Hence in present research investigation, Mn3O4 powder was exposed to Mr. Trivedi’s biofield in order to improve its physical, structural, and magnetic properties. The treated Mn3O4 samples were characterized by FT-IR, XRD, ESR, Brunauer-Emmett-Teller (BET) analysis and particle size analysis.


Manganese (II, III) oxide powders used in the present investigation were obtained from Sigma Aldrich, USA (97% in purity). Five sets of these metal oxide powders were prepared from the master sample, where first set was considered as control which was untouched (unexposed), other four samples were exposed to Mr. Trivedi’s biofield, referred herein as treated sample (T1, T2, T3, and T4).

Particle size of control and treated samples were measured by laser particle size analyzer, SYMPATEC HELOS-BF, had a detection range of 0⋅1-875μm with setting parameters remainthe same for all evaluations. The data obtained from particle size analyzer was in the form of a chart of cumulative percent vs. particle size. The surface area of all samples was measured by surface area analyzer SMART SORB 90 Brunauer-Emmett-Teller (BET). For atomic and structural level analysis, all samples were characterized by X-ray diffraction (XRD) [Phillips, Holland PW 1710] whichhas used copper anode with nickel filter and the wavelength of the radiation 1.54056 Å. The Data obtained from the XRD system was in the form of a chart of 2θ vs. intensity with a detailed table containing peak intensity counts, d, value (Å), peak width (θ0), relative intensity (%). The‘d’ values were compared with a database of standard JCPDS (Joint Committee on Powder Diffraction Standards). Lattice parameter and unit cell volume was obtained by using PowderX software. Crystallite size was computed as:

Crystallite size = [kλ/(bCosθ)]

Where λ is the wavelength of x-radiation used (1.54056 x 10-10 m) and k is the equipment constant (0.94).

The molecular weight of atom calculated as:

Molecular weight= number of protons x weight of a proton + number of neutrons x weight of a neutron + number of electrons x weight of an electron.

Molecular weight in g/Mol was calculated from the weights of all atoms in a molecule multiplied by the Avogadro number (6.023 x 1023). As number of molecules per unit cell is known so the weight of unit cell can be computed easily by multiplying molecular weight to number of molecules per unit cell. Density was computed as the ratio of the weight of the unit cell to the volume of the unit cell. Micro strain and dislocation density were calculated[9] as: Micro strain = [bcosθ/4]

Dislocation density = [1/ (Crystallite size) 2]

Percentage change in lattice parameter was calculated as:

% change in lattice parameter = 100 x (Δa/ac)

Where Δa is the difference in lattice parameter of control and treated powders and ac is the lattice parameter of control powder. Percent change in volume, molecular weight, density, micro strain; dislocation density was computed in a similar manner. IR spectra were evaluated using Perkin Elmer, Fourier Transform Infrared (FT-IR) Spectrometer, in the range of 300-4000 cm-1. Paramagnetic properties were characterized by Electron Spin Resonance (ESR), E-112 ESR Spectrometer of Varian USA of X-band microwave frequency (9.5 GHz), which had sensitivity of 5 x 1010, ΔH spins.

No. of days After Treatment Control Sample Day 1 Treated powder after 11 Days (T1) Treated powder after 85 Days (T2) Treated powder after 99 Days (T3) Treated powder after 105 Days (T4)
Average particle Size d50 (µm) 6.1 6.9 5.9 6.1 6.1
Percent change in Average Particle size (d50) 13.1 -3.3 0 0
d99, Size below which 99% particles present (µm) 31.1 29.4 28.4 28.4 29
Percent change in particle size d99(%) -5.5 -8.7 -8.7 -6.8

Table 1. Particle size of control and treated sample of Mn3O4.

No. of days After Treatment Control 11 85 90
Surface Area (m2/g) 3.08 2.95 2.95 2.77
Percentage Change in Surface Area (%) -4.083 -4.259 -9.951

Table 2.
Surface area result of control and treated sample of Mn3O4 after biofield treatment


Results and Discussions:

Particle Size and Surface Area Analysis

The particle size determination of ceramic materials provides superior control over a range of product performance characteristics. The particle size of Mn3O4 was determined and illustrated in Figure 1. The average particle size (d50Od50) in treated sample was increased upto13% and then further was decreased by 3%.

Contrarily particle size d99 (size below which 99% particles present) was reduced by 5.5% in treated Mn3O4 samples. Surface area of the Mn3O4 was measured by using BET analysis, and results are presented in Figure 2 and Table 2. The Surface area of treated powders were reduced by 10% in 99 days after biofield treatment. Initially surface area were decreased by 4.5% with corresponding increase in particle size, however after 80 days both surface area and particle size were reduced. The particle size was increased initially, which was supported by a decrease in surface area due to the agglomeration of fine particles. Nevertheless a decrease in both particle size and surface area after 80 days indicate that coarse particles would have fractured into finer particles with sharp edges and corners.

Figure 1

Figure 2

Figure 3

Figure 4

Figure 4

Figure 6

Figure 7

X-ray diffraction (XRD)

Mn3O4 ceramic powder was subjected to XRD analysis to investigate its crystalline nature and Powder X software was used to calculate various atomic and structural parameters. The XRD diffractogram of control and treated Mn3O4 samples are illustrated in Figure 3(a-e). In the XRD diffractogram, only Mn3O4 phase appears with intense crystalline peaks (JCPDS Card No. 0041-1442) at Braggs angle 2θ = 17.8o, 28.7 o, 32.2 o, 36 o, 37.8 o, 44.2 o, 50.4 o, 58.2 o, 59.6 o, 64.6 o, 73.8 o. These crystalline peaks are attributed to plane (101), (112), (103), (211), (004), (220), (105), (321), (224), (400) and (413) respectively. The intensity of peaks increased in treated Mn3O4 samples along (103), (211), and (224) direction confirming increased crystallinity in treated samples(Figure 3 b-e). This result indicates that biofield treatment is directly acting upon the ceramic crystals inducing more long range order; thereby facilitating crystallization of the ceramic samples.

Figure 4 shows that the lattice parameter was reduced in treated samples from 0.25% to -0.30% in time period of 16 to 147 days. It was found that reduction in lattice parameter caused reduction in volume of unit cell and increase in density (Figure 4). Additionally molecular weight was decreased by around -0.50 to -0.60 % in treated Mn3O4 samples in 147 days. The crystallite size was calculated from the XRD graph and the results are presented in Figure 5. The crystallite size was significantly enhanced by 96% in treated Mn3O4 samples in 147 days, which could be due to the reorientation of the planes in the same direction and unhindered movements of dislocations across grain boundaries, which causes reduction of dislocation density by 50% (Figure 1). Nevertheless the movement of dislocations needs large amount of energy, so it is believed that energy used for this process was provided by two different sources: biofield and the energy released during conversion of mass(as per Einstein energy equation E=mc2). This fact was well supported by loss in molecular weight of treated Mn3O4 sample.

The large difference in crystallite size and particle size can be explained by the cumulative effect of fracturing, agglomeration and consolidation process induced by energy milling through biofield treatment. Moreover the noticeable decrease in micro strain and dislocation density also supports the above observation (Figure 5).

Control Sample Day 1 16 Days after treatment (T1) 106 Days after treatment (T2) 131 Days after treatment (T3) 147 Days after treatment (T4)
Lattice parameter (a) (A0) 5.810984 5.796193526 5.797734 5.796267 5.793405
Percent Change in Lattice Parameter (%) -0.25452492 -0.22802 -0.25326 -0.30251
Volume of unit cell (A0) 3.17662 3.16047 3.16215 3.16055 3.15743
Percent Change in volume (%) -0.50840201 -0.45552 -0.50588 -0.6041
Density 4.828738 4.853413148 4.850835 4.85329 4.858086
Percent Change in Density (%) 0.510999946 0.4576 0.508456 0.607773
Molecular weight (g/mol) 233.4512 232.2643677 232.3878 232.2702 232.041
Percent Change in Molecular weight (%) -0.50840201 -0.45552 -0.50588 -0.6041
Crystalline Size (nm) 87 108.8 108.8 145.03 170.76
Percent Change in Crystalline Size (%) 20.04800909 20.03084 50.02997 96.28
Micro Strain 0.000416 0.00033275 0.000333 0.00025 0.000212
Percent change in Micro Strain (%) -20.0367647 -20.0368 -40.0124 -49.0513
Dislocation Density (lines/m2) *1015 0.132118 0.084477725 0.084478 0.047543 0.034295
Percentage change in Dislocation density (%) -36.05881 -36.0588 -64.0149 -74.0423

Table 3. Atomic and crystal structure characteristics of Mn3O4, computed result from XRD.

Figure 8

Figure 9

FT-IR Spectroscopy

The FT-IR spectra of control and treated Mn3O4 samples are presented in Figure 6 (a and b). The FT-IR of control sample showed vibration peak at 651 cm-1 that corresponds[33] to Mn-O stretching in tetrahedral and 563 cm-1 corresponds to Mn+3 -O in octahedral positions. Other important peaks were observed at 3500 cm-1 and 1500 cm-1 which were attributed to weakly bound moisture (water molecules) in treated and control samples[33].

Figure 10

Figure 11

In Figure 6(a), it was found that the treated sample T1 has not showed any peak in the fingerprint region 450-700 cm-1, which was quite unexpected. It can be hypothesized that Mn-O bond was no longer exists, or strength of Mn-O bond was greatly reduced. Contrarily treated sample T2 showed intense absorption peaks at 557cm-1 and 613cm-1 which was responsible to Mn-O in octahedral and Mn+3-O in tetrahedral position respectively (Figure 6b). It was also noticed that vibration peaks were shifted to lower wavenumber as compared to control sample that indicates that Mn-O bond length was reduced (Figure 6b). Therefore, IR spectra revealed that Mn-O bond length and bond force constant was significantly altered by biofield.

Electron Spin Resonance (ESR) Spectroscopy

The ESR spectra analysis result of control and treated Mn3O4 samples are illustrated in Figure 7. It was found that the g-factor was slightly increased by 0.15%, which indicated that the angular momentum of the electrons in the atom was probably increased through biofield treatment. It was also observed that the spin resonance signal width of the treated sample was broadened by 11%, which could be due to the increase in dipole-dipole and electrostatic interaction among Mn ions with other mixed valance states[34-35]. Additionally, the resonance signal peak intensity was increased by 16% that might be due to the clustering of spins on the particle surface, that may led to enhanced the magnetisation of treated Mn3O4 samples. This result was also supported by increase in crystallinity and particle size [9]. Further it was hypothesized that during high energy milling through biofield treatment, spins may get clustered on the surface and enhanced the magnetisation. Furthermore, particle size analysis showed increase in particle size which is associated with the increase in volume of individual particles. Further, the increase in volume of individual particle led to enhanced the magnetic moment17 in individual particles of treated Mn3O4[17].

Figure 12


Current research work investigates the modulation of crystalline, physical, atomic and magnetic properties of Mn3O4 ceramic powders using Mr. Trivedi‘s biofield. The particle size of Mn3O4 powder was increased after biofield treatment, which results into reduced surface area, which may be due to combine effect of rupturing and agglomeration process.

XRD result demonstrated that biofield had significantly reduced the unit cell volume by 0.60%, that was probably due to compressive stress applied during energy milling. Biofield exposed sample showed the larger crystalline size as compared to control Mn3O4, which was mainly due to reduction of the dislocation density and microstrain cause reorientation of neighbouring planes in same direction and thereby increasing crystallite size. The reduction in dislocation density and microstrain could have led to enhance the paramagnetic behaviour of Mn3O4.

ESR results revealed that magnetization and spin-spin atomic interaction of treated sample was enhanced, which may be due to increasing in spin cluster density and high crystallinity respectively. Hence the increase in spin cluster density could led to enhance the magnetisation of Mn3O4 nanopowders.These excellent results indicates that biofield treated Mn3O4 ceramic powders can be used as novel materials for fabricating magnetic data storage devices and future research is needed to explore its further applications.


We would like to give thanks to all the staff of various laboratories for supporting us in conducting experiments.Special thanks to Dr Cheng Dong of NLSC, Institute of Physics and Chinese academy of sciences for providing the facilities to use PowderX software for analyzing XRD results.


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