6th Anti-Infectives Partnering & Deal-Making Summit on March 5-6, 2009 in Boston, MA

Date: March 5-6, 2009, Location: Boston , Massachusetts- USA

Presentation: Oral

 

Title: Targeted Impact Of External Energy on Plasma Viral Load of Hepatitis C Virus In Vitro

Abstract Accepted:

Background

The objective was to observe the targeted impact of external energy on plasma viral loads (PVL) of Hepatitis C virus (HCV).

 

Methods

In vitro, experiments were carried out on positive HCV samples being in plasma state throughout the investigation. All HCV (PVL) samples were analyzed before they were exposed to the external energy, which were considered being control.

After these samples were exposed to the external energy, they were analyzed again and reported as treated. Experiments were carried out in the Microbiology Laboratory of P.D. Hinduja National Hospital & Medical Research Centre, Mumbai, India– accredited by The College of American Pathologists. While exposing these samples to said energy, optimum precautions to maintain the cold chain were taken.

 

Results

Analyzed data showed changes in viral loads to considerable extent. In one case the viral load decreased by ≥99.81% within 10 days, achieving clinically significant change of over ≥2.71 log, as opposed to that obtainable over more than 4-6 weeks with the best possible conventional therapy.

Significant increases have also been observed up to 151% within 10 days.

 

Conclusions

PVL of HCV is not normally expected to either increase or decrease when stored at temperatures below  -70 oC. But in the present study, significant changes have been observed within 10 days in vitro which suggest the influence of an external energy that has affected HCV viral loads causing both decrease and increase to significant level. The targeted increase in plasma itself suggests that there could be other factors contributing to the replication of HCV in plasma as opposed to the replication in the host cells. Additionally, an alternative model for replication of HCV in plasma has been suggested.