Wound Healing Potential of Biofield Energy Treatment in HaCaT Cell Line Using Scratch Assay

Journal: Global Research Journal of Public Health and Epidemiology PDF  Web

Published: 28th September, 2018 Volume: 5 Issue: 5 Pages: 136-142

DOI: ISSN: 2360-7920

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar and Snehasis Jana

  • 2560 Views
  • 1359 Downloads

Abstract

Wounds may be acute, subacute, or chronic and are the common clinical life entities. It is a complex biochemical process to restore the cell structures that depends upon cell proliferation and migration, mainly by keratinocytes and fibroblast cells. The aim of the present study was to evaluate the effect of the Biofield Energy Treatment (Consciousness Energy Healing Treatment-The Trivedi Effect®) in the HaCaT cell line (Human keratinocytes) and DMEM (Dulbecco’s Modified Eagle Medium) for wound healing action using scratch assay (48 hours) against positive control i.e., recombinant Human Epidermal Growth Factor (Hu-EGF, 30 ng/mL). In vitro scratch assay monitored proliferation and migration of keratinocytes in the scratched wounded area using WimScratch Image analysis software. The results showed that the Biofield Energy Treated DMEM group have significantly higher percentage of keratinocytes migration i.e., 35.4%, while no migration was monitored in the Biofield Energy Treated HaCaT cells as compared with the baseline control group. Additionally, the percentage of scratch area was significantly decreased by 7.3% in the Biofield Energy Treated DMEM, while an increased scratched area was reported by 14% in the Biofield Energy Treated cells. Hence, the results using scratch assay showed a promising scientific approach to differentiate the Biofield Energy Treatment on cells or media for their wound healing potential. The data concluded that The Trivedi Effect® has the significant capability in wound healing activity in DMEM as compared with the HaCaT cell line with respect to the cell migration and reduced wound scratched area. Consciousness Energy Healing Treatment (The Trivedi Effect®) can be used as a complementary and alternative approach to improve the cellular migration and proliferation and can be used in psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections as anti-wrinkling, skin-whitening, anti-ageing, and rejuvenating agent.

Conclusion

In the present experimental, an established in vitro wound healing model was used in order to elucidate the potential use of Biofield Energy Healing (The Trivedi Effect®) on DMEM and HaCaT cells in 48 hours study period. The cell migration analysis showed 73.5% cellular migration rate in EGF group, while Biofield Energy Treated DMEM was observed with 35.4% migration rate, while no migration was reported in Biofield Energy Treated cells as compared with the baseline control group. Further, the percentage of scratch area was significantly reduced by 7.3% in the Biofield Energy Treated DMEM, while it was increased by 14% in the Biofield Energy Treated cells. However, positive control EGF showed 3% scratched area, suggest significant wound healing. Overall, study results conclude that Mr. Trivedi’s Biofield Energy Treatment has the capacity to improve the wound healing rate in HaCaT cells using Biofield Energy Treated DMEM media through an improved keratinocytes migration rate results in improved wound healing potential. Hence, The Trivedi Effect® can be used as a Complementary and Alternative Medicine (CAM) in cell culture media as an alternate treatment approach for wound healing along with other skin-related disorders such as eczema, diaper rash, chickenpox, measles, warts, acne, hives, wrinkles, ringworm, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections, rashes from allergic reactions, raised bumps that are red or white, cracked skin, discolored patches of skin, fleshy bumps, warts, or other skin growths, changes in mole color or size, a loss of skin pigment, scaly or rough skin, peeling skin, ulcers, open sores or lesions, dry, excessive flushing.