Journal: Biomarkers & Applications PDF
Published: 25 June, 2018 Volume: 2018 Issue: 2 Pages: 1-9
DOI: 10.29011/2576-9588. 100025 ISSN: 2576-9588
Authors: James Jeffery Peoples, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana
Citation: Peoples JJ, Mahendra MK, Branton A, Trivedi D, Nayak G, et al. (2018) The Bone Regenerative Effects of the Biofield Energy Treated Vitamin D3 in Human Bone Osteosarcoma Cells (MG-63). Biomark Applic: BMAP-125. DOI: 10.29011/2576-9588. 100025
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The current research work was presented to evaluate impact of Biofield Energy Treated vitamin D3 and DMEM on bone health in human bone osteosarcoma cells (MG-63). The Test Items (TI), were distributed into two parts. One part of each sample was received Consciousness Energy Healing Treatment by James Jeffery Peoples and labeled as Biofield Energy Treated (BT) samples, while other parts of each sample were denoted as Untreated Test Items (UT). Test samples were found as safe in tested concentrations by MTT assay. ALP was significantly increased by 54.43% and 111.24% at 10 and 100 µg/mL, respectively in BT-DMEM + UT-TI than UT-DMEM + UT-TI. Moreover, ALP was significantly elevated by 128.14%, 77.84%, and 62.28% in UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI, respectively at 1 µg/mL compared to untreated. Collagen was significantly increased by 286.67% and 340% in BT-DMEM + UT-TI and BT-DMEM + BT-TI, respectively at 0.1 µg/mL, while increased by 134.08% in UT-DMEM + BT-TI at 10 µg/mL than untreated. Besides, percent of bone mineralization was remarkably increased by 140.94%, 113.72%, and 129.87% at 1 µg/mL in UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI, respectively, while increased by 187.91% in BT-DMEM + UT-TI at 0.1 µg/mL than untreated. Altogether, Biofield Treated vitamin D3 was significantly improved the bone growth and it could be able to fight against various bone-related disorders (osteoporosis, osteogenesis imperfecta, Paget’s disease, rickets, osteomalacia), autoimmune and inflammatory diseases, stress management, and anti-aging by improving overall health.
The MTT cell viability assay data showed greater than 71% cells were viable, which indicated that the test samples were safe and nontoxic in the selected concentrations. The UT-DMEM + BT-Test item group exhibited 128.14% increased the level of ALP at 1 µg/mL than untreated. Moreover, the BT-DMEM + UT-Test item group revealed the percent increased of ALP by 77.84%, 54.43%, and 111.24% at 1, 10, and 100 µg/mL, respectively as compared to the untreated group. Other parameter like collagen was significantly increased by 286.67% and 340% in the BT-DMEM + UT-TI and BT-DMEM + BT-TI, respectively at 0.1 µg/mL, while increased by 134.08% in the UT-DMEM + BT-Test item group at 10 µg/mL compared to the untreated group. Besides, the percent of bone mineralization was remarkably increased by 140.94%, 113.72%, and 129.87% at 1 µg/mL in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively, while increased by 187.91% in the BT-DMEM + UT-Test item group at 0.1 µg/mL compared to the untreated group. In conclusion, Biofield Treated (The Trivedi Effect®) test samples demonstrated a significant impact on bone health parameters. Therefore, the Consciousness Energy Healing-based vitamin D3 might be use for the management of bone-related disorders viz. low bone density and osteoporosis, rickets, osteogenesis imperfecta, bone and joint pain, osteoma, osteomalacia, bone fractures, etc. Further, it can used in organ transplants (kidney, liver, and heart transplants), autoimmune disorders (Systemic Lupus Erythromatosus, Addison Disease, Celiac Disease or gluten-sensitive enteropathy, Graves’ Disease, Hashimoto Thyroiditis, Multiple Sclerosis, Myasthenia Gravis, Pernicious Anemia, Aplastic Anemia, Rheumatoid Arthritis, Type 1 Diabetes, Alopecia Areata, Crohn’s Disease, Fibromyalgia, Vitiligo, Psoriasis, Scleroderma, Chronic Fatigue Syndrome and Vasculitis), and inflammatory disorders (Asthma, Ulcerative Colitis, Alzheimer’s Disease, Atherosclerosis, Dermatitis, Diverticulitis, Hepatitis, Irritable Bowel Syndrome). Further, it could be useful for the management of stress, cancer, psychosis, fibrosis, aging, neurodegenerative disorders (Parkinson’s), wound repair and improve Quality of Life.