Journal: Journal of Emergency Medicine & Critical Care PDF
Published: 06 July, 2018 Volume: 4 Issue: 1 Pages: 01-06
DOI: 10.13188/2469-4045.1000015 ISSN: 2469-4045
Authors: Julia Grace McCammon, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal and Snehasis Jana
Citation: McCammon JG, Trivedi MK, Branton A, Trivedi D, Nayak G, et al. Role of Biofield Energy Treated Vitamin D3 in Human Bone Osteosarcoma Cells (MG-63): A Multidisciplinary Aspect on Bone Health. J Emerg Med Critical Care 2018;4(1): 6.
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Study the impact of Biofield Treated vitamin D3 and DMEM on bone health in human bone osteosarcoma cells (MG-63) was investigated. The test items (TI), were distributed into two parts. One part of each sample was received the Consciousness Energy Healing Treatment by Julia Grace McCammon, and those samples were labeled as Biofield Treated (BT) samples, while other parts of each sample were denoted as untreated test items (UT), where did not provide any types of treatment. Cell viability showed test samples were found as safe in tested concentrations. ALP was significantly increased by 210.4% and 221.6% in UT-DMEM+BT-TI and BT-DMEM+BT-TI respectively at 0.1 µg/mL, while increased by 207.9% in BT-DMEM+UT-TI compared to UT-DMEM+UT-TI. Collagen was significantly increased by 158.46%, 129.23%, and 138.46% in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI respectively at 1 µg/mL compared to untreated. Moreover, level of collagen was significantly enhanced by 101.37%, 157.53%, and 176.71% in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI respectively at 10 µg/mL compared to untreated. Besides, the percent of bone mineralization was remarkably increased by 70.59% and 151.18% in the BT-DMEM+UT-TI and BT-DMEM+BT-TI respectively at 100 µg/mL, while increased by 117.17% in UT-DMEM+BT-TI at 1 µg/mL than untreated. Altogether, the Biofield Energy Treated vitamin D3 was significantly improved the bone cell growth-related parameters. It could be used as an alternative supplement for vitamin D3 deficiency on various bone-related disorders (osteoporosis, low bone density, Paget’s disease, rickets, osteomalacia), stress, aging, autoimmune, and inflammatory disorders.
The cytotoxicity assay (MTT) data showed more than 73% cells were viable, which indicated the test samples were safe and nontoxic in the tested concentrations. ALP was significantly elevated by 210.4% and 221.6% in the UT-DMEM+BT-TI and BT-DMEM+BT-TI, respectively at 0.1 µg/mL, however increased by 207.9% in the BT-DMEM+UT-TI at 50 µg/mL than UT-DMEM+UT-TI. Collagen was significantly increased by more than 100% in all the treatment groups at different concentrations than untreated. Besides, the percent of bone mineralization was remarkably increased by 151.18% in the BT-DMEM+BT-TI at 100 µg/mL, while increased by 117.17% in the UT-DMEM+BT-TI at 1 µg/mL compared to the untreated group. Altogether, the Biofield Treated test items possess a significant impact on bone health parameters. Thus, the Consciousness Energy Healing-based vitamin D3 might be suitable for the development of an alternative supplement in vitamin D3 deficiency cases, which could be used for the management of various bone-related disorders viz. Paget’s disease, osteogenesis imperfecta, osteoporosis, osteomalacia, rickets, bone fractures, bone and joint pain, deformed bones, etc. Moreover, it can also be utilized on organ transplants (liver, kidney, and heart transpant), autoimmune disorders (Myasthenia Gravis, Addison Disease, Dermatomyositis, Aplastic Anemia, Pernicious Anemia, Graves’ Disease, Hashimoto Thyroiditis, Multiple Sclerosis, different types of Arthritis, Systemic Lupus Erythematosus, Type 1 Diabetes, Vitiligo, Crohn’s Disease, Fibromyalgia, Psoriasis, Scleroderma, Vasculitis). It can also be useful against an inflammatory disorders (irritable bowel syndrome, ulcerative colitis), anti-aging Atherosclerosis, wound healing, anti-stress, anti-cancer, and Parkinson’s disease, etc. near future.