Protective effects of tetrahydrocurcumin (THC) on fibroblast and melanoma cell lines in vitro: it’s implication for wound healing

Journal: Journal of Food Science and Technology PDF  

Published: 27-Feb-17 Volume: 54 Issue: 5 Page Number: 1137–1145

DOI: 10.1007/s13197-017-2525-8 ISSN: 0022-1155 (Print) 0975-8402 (Online)

Authors: Mahendra Kumar Trivedi, Mayank Gangwar, Sambhu Charan Mondal, Snehasis Jana

Citation: Trivedi, M.K., Gangwar, M., Mondal, S.C. et al. J Food Sci Technol (2017) 54: 1137.

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The aim of this study was to investigate the role of tetrahydrocurcumin (THC) against various skin health parameters using in vitro human foreskin fibroblast and melanoma cell lines (i.e. HFF-1 and B16-F10). The study was assessed using cell viability by MTT assay, identification of extracellular matrix component in HFF-1 cell line (i.e. collagen, elastin and hyaluronic acid), melanin synthesis in B16-F10 cells, cell viability against UVB-induced stress in HFF-1 cells, and in vitro wound healing by the scratch assay. THC was found to be safe and nontoxic up to the concentration of 10 µg/mL with improved level of collagen (37.90%), elastin (90.1%), and hyaluronic acid (74.19%) at 1 µg/mL. Besides, melanin was significantly inhibition by 78.5% at the lowest THC concentration of 0.1 µg/mL. UVB-protection rate was significantly improved by 61.2% and improved cell viability by THC in HFF-1 cells, which indicated protection from photoaging. In addition, THC showed significant wound healing activity (78.51%) and greater migration of fibroblast in HFF-1 cells at different time period. It can be concluded from the study that THC can protect the skin matrix with improved extracellular component synthesis and would healing via collagen synthesis in the skin, which improved the skin elasticity and tightness. Overall, it might be suggested that THC can be used as a safe skin whitening agent, wounds management, cosmetic applications, and treating various skin-related disorders.


B16-F10   Extracellular matrix   HFF-1   Hyaluronic acid   Scratch assay   Tetrahydrocurcumin



The authors extend their sincere thanks and grateful to Dabur Research Foundation, India for providing the facilities and support that enabled the successful completion of the work. Further, the authors report no conflicts of interest in this work. However, no external funding is associated with this study.

Compliance with ethical standards

Conflict of interest

The authors state no conflict of interest.

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