Modulation of Pro-inflammatory Cytokines Expression of the Biofield Energy Healing (The Trivedi Effect®) Based Herbomineral Formulation in Mouse Splenocytes

Journal: International Journal of Biomedical Engineering and Clinical Science PDF  

Published: 7-Jan-17 Volume: 2 Issue: 1 Pages: 8 – 17

DOI: 10.11648/j.ijbecs.20160201.12 ISSN: 2472-1298 (Print) 2472-1301 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, William Dean Plikerd, Peter L. Surguy, Robert John Kock, Rolando Baptista Piedad, Russell Phillip Callas, Sakina A. Ansari, Sandra Lee Barrett, Sara Friedman, Steven Lee Christie, Su-Mei Chen Liu, Susan Elizabeth Starling, Susan Jones, Susan Mardis Allen, Susanne Kathrin Wasmus, Terry Ann Benczik, Thomas Charles Slade, Thomas Orban, Victoria L. Vannes, Victoria Margot Schlosser, Yusif Sarkis Yamin Albino, Sambhu Charan Mondal, Snehasis Jana

Citation: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, William Dean Plikerd, Peter L. Surguy, Robert John Kock, Rolando Baptista Piedad, Russell Phillip Callas, Sakina A. Ansari, Sandra Lee Barrett, Sara Friedman, Steven Lee Christie, Su-Mei Chen Liu, Susan Elizabeth Starling, Susan Jones, Susan Mardis Allen, Susanne Kathrin Wasmus, Terry Ann Benczik, Thomas Charles Slade, Thomas Orban, Victoria L. Vannes, Victoria Margot Schlosser, Yusif Sarkis Yamin Albino, Sambhu Charan Mondal, Snehasis Jana. Modulation of Pro-inflammatory Cytokines Expression of the Biofield Energy Healing (The Trivedi Effect®) Based Herbomineral Formulation in Mouse Splenocytes. International Journal of Biomedical Engineering and Clinical Science. Vol. 2, No. 1, 2016, pp. 8-17. doi: 10.11648/j.ijbecs.20160201.12

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Abstract

With the increasing popularity of herbomineral preparations in healthcare, a new proprietary herbomineral formulation was formulated with ashwagandha root extract and three minerals viz. zinc, magnesium, and selenium. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the herbomineral formulation using murine splenocyte cells. The test formulation was divided into two parts. One was the control without the Biofield Energy Treatment. The other part was labelled the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers. Through MTT assay, all the test formulation concentrations from 0.00001053 to 10.53 µg/mL were found to be safe with cell viability ranging from 102.61% to 194.57% using splenocyte cells. The Biofield Treated test formulation showed a significant (p≤0.01) inhibition of TNF-α expression by 15.87%, 20.64%, 18.65%, and 20.34% at 0.00001053, 0.0001053, 0.01053, and 0.1053, µg/mL, respectively as compared to the vehicle control (VC) group. The level of TNF-α was reduced by 8.73%, 19.54%, and 14.19% at 0.001053, 0.01053, and 0.1053 µg/mL, respectively in the Biofield Treated test formulation compared to the untreated test formulation. The expression of IL-1β reduced by 22.08%, 23.69%, 23.00%, 16.33%, 25.76%, 16.10%, and 23.69% at 0.00001053, 0.0001053, 0.001053, 0.01053, 0.1053, 1.053 and 10.53 µg/mL, respectively compared to the VC. Additionally, the expression of MIP-1α significantly (p≤0.001) reduced by 13.35%, 22.96%, 25.11%, 22.71%, and 21.83% at 0.00001053, 0.0001053, 0.01053, 1.053, and 10.53 µg/mL, respectively in the Biofield Treated test formulation compared to the VC. The Biofield Treated test formulation significantly down-regulated the MIP-1α expression by 10.75%, 9.53%, 9.57%, and 10.87% at 0.00001053, 0.01053, 0.1053 and 1.053 µg/mL, respectively compared to the untreated test formulation. The results showed the IFN-γ expression was also significantly (p≤0.001) reduced by 39.16%, 40.34%, 27.57%, 26.06%, 42.53%, and 48.91% at 0.0001053, 0.001053, 0.01053, 0.1053, 1.053, and 10.53 µg/mL, respectively in the Biofield Treated test formulation compared to the VC. The Biofield Treated test formulation showed better suppression of IFN-γ expression by 15.46%, 13.78%, 17.14%, and 13.11% at concentrations 0.001053, 0.01053, 0.1053, and 10.53 µg/mL, respectively compared to the untreated test formulation. Overall, the results demonstrated that The Trivedi Effect®– Biofield Energy Healing (TEBEH) has the capacity to potentiate the immunomodulatory and anti-inflammatory activity of the test formulation. Biofield Energy may also be useful in organ transplants, anti-aging, and stress management by improving overall health and quality of life.

Conclusion

Cell viability was assessed using MTT assay and the herbomineral formulation was found safe at all the tested concentrations. The level of TNF-α was significantly decreased by 19.54% and 14.19% at 0.01053 and 0.1053 μg/mL, respectively in the Biofield Energy Treated test formulation as compared to the untreated test formulation. Moreover, the level of IL-1β was significantly reduced by 6.90% and 14.71% at 0.1053 and 10.53 μg/mL, respectively in the Biofield Energy Treated test formulation as compared to the untreated test formulation. The expression of MIP-1α was significantly reduced by 10.75%, 9.53%, 9.57%, and 10.87% at 0.00001053, 0.01053, 0.1053 and 1.053 μg/mL, respectively in the Biofield Energy Treated test formulations as compared to the untreated test formulation. Furthermore, the Biofield Energy Treated test formulations showed 15.46%, 13.78%, 17.14%, and 13.11%, down-regulation of IFN-γ expression at 0.001053, 0.01053, 0.1053 and 10.53 μg/mL, respectively as compared to the untreated test formulation.

Therefore, The Trivedi Effect® Biofield Energy Healing (TEBEH) administered remotely by the twenty Biofield Energy Healers enhanced the herbomineral test formulation’s anti-inflammatory and immunomodulatory properties. Overall, Biofield Energy Treated test formulation significantly inhibited the activity of pro-inflammatory cytokines and showed immunosuppressive activity with a safe therapeutic index as compared with the untreated test formulation. Biofield Energy Healing shows great promise as a complementary and alternative medicine therapy and might play an important role in autoimmune diseases and inflammatory disorders like Celiac Disease (gluten-sensitive enteropathy), Irritable Bowel Syndrome, Parkinson’s Disease, Graves’ Disease, Chronic peptic ulcers, Hepatitis, Addison Disease, Multiple Sclerosis (MS), Tuberculosis, Rheumatoid arthritis, Chronic periodontitis, Crohn's disease, Ulcerative colitis, Lupus, Systemic Lupus Erythematosus, Vitiligo, Hashimoto Thyroiditis, Chronic sinusitis, Type 1 Diabetes, Asthma, Chronic active hepatitis, Pernicious and Aplastic Anemia, Vasculitis, Myasthenia Gravis, Rheumatoid Arthritis, Reactive Arthritis, Sjogren Syndrome, Alopecia Areata, Scleroderma, Dermatitis, Psoriasis, Fibromyalgia, Diverticulitis, Chronic Fatigue Syndrome, Alzheimer’s Disease, Atherosclerosis, stress, etc. Biofield Energy Healing may also be useful in the prevention of immune-mediated tissue damage in cases of organ transplants (for example heart transplants, liver transplants and kidney transplants), for anti-aging and in improving overall health and quality of life.