Journal: Journal of Solution Chemistry Web
Published: 10-Jan-17 Volume: 46 Issue: 4 Pages: 896907
DOI: 10.1007/s10953-017-0613-z ISSN: 0095-9782 (Print) 1572-8927 (Online)
Authors: Mahendra Kumar Trivedi, Parthasarathi Panda, Kalyan Kumar Sethi, Snehasis Jana
Citation: Trivedi, M.K., Panda, P., Sethi, K.K. et al. J Solution Chem (2017) 46: 896. https://doi.org/10.1007/s10953-017-0613-z
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Magnesium gluconate is a classical pharmaceutical compound used as a source of magnesium for the prevention and treatment of hypomagnesemia. To the best of our knowledge, a robust and reliable liquid chromatography tandem mass spectrometry technique has not yet been reported for the qualitative and quantitative analysis of magnesium gluconate. This study describes the method development for the LCESIMS/MS analysis of magnesium gluconate using three different reversed-phase HPLC conditions (Method IIII) with comprehensive fragmentation pattern and the structural characterization by NMR spectroscopy. The LCMS and NMR data were found in accordance with the structure of magnesium gluconate. When magnesium gluconate was dissolved in the acetonitrile and watermethanol solutions, it exists in situ in three different forms: magnesium gluconate itself, gluconic acid, and magnesium gluconate chelate with gluconic acid by a coordinate covalent bond. Method I exhibited pseudo-molecular ion peaks with more magnesium gluconate chelates with gluconic acid, while method II showed an adduct of magnesium gluconate with the solvent along with the molecular ion peak. There was no pseudo-molecular ion peaks found in method III. Thus, method III was found to be the more accurate, robust and reliable LCMS method for the qualitative and quantitative analysis, structural characterization, and could also be suitable for the pharmacokinetic study of magnesium gluconate. The detailed fragmentation analysis might be useful for the structural characterization of unknown divalent organometallics.