Investigation of Immune Biomarkers Regulation by Biofield Energy Healing (The Trivedi Effect®) Based Herbomineral Formulation in Male Sprague Dawley Rats

Journal: Cell Biology PDF  

Published: 11-Dec-17 Volume: 5 Issue: 6 Pages: 66-75

DOI: 10.11648/j.cb.20170506.12 ISSN: 2330-0175 (Print) 2330-0183 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, William Dean Plikerd, Peter L. Surguy, Robert John Kock, Rolando Baptista Piedad, Russell Phillip Callas, Sakina A. Ansari, Sandra Lee Barrett, Sara Friedman, Steven Lee Christie, Su-Mei Chen Liu, Susan Elizabeth Starling, Susan Jones, Susan Mardis Allen, Susanne Kathrin Wasmus, Terry Ann Benczik, Thomas Charles Slade, Thomas Orban, Victoria L. Vannes, Victoria Margot Schlosser, Yusif Sarkis Yamin Albino, Mayank Gangwar, Snehasis Jana

Citation: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, William Dean Plikerd, Peter L. Surguy, Robert John Kock, Rolando Baptista Piedad, Russell Phillip Callas, Sakina A. Ansari, Sandra Lee Barrett, Sara Friedman, Steven Lee Christie, Su-Mei Chen Liu, Susan Elizabeth Starling, Susan Jones, Susan Mardis Allen, Susanne Kathrin Wasmus, Terry Ann Benczik, Thomas Charles Slade, Thomas Orban, Victoria L. Vannes, Victoria Margot Schlosser, Yusif Sarkis Yamin Albino, Mayank Gangwar, Snehasis Jana. Investigation of Immune Biomarkers Regulation by Biofield Energy Healing (The Trivedi Effect®) Based Herbomineral Formulation in Male Sprague Dawley Rats. Cell Biology. Vol. 5, No. 6, 2017, pp. 66-75. doi: 10.11648/j.cb.20170506.12

  • 3450 Views
  • 1468 Downloads

Abstract

Herbomineral formulations have been used worldwide against various chronic and degenerative diseases, due to its fewer side effects. A new proprietary herbomineral formulation was formulated, consisting of essential ingredients viz. herbal root extract of ashwagandha and minerals (zinc, magnesium, and selenium). The present study aimed to evaluate the impact of Biofield Energy Treated herbomineral formulation in male Sprague Dawley (SD) rats for immune biomarkers modulation. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Treated sample, which received the Biofield Energy Healing Treatment remotely from twenty renowned Biofield Energy Healers. The immunomodulatory biomarkers like alteration in immune biomarkers such as IgG and IgM, CD4+ and CD8+, hematology, lipid profile, hepatic enzymes, sex hormone, and antioxidant profile. The humoral immune response of IgM and IgG were altered by 4.54% and 9.49%, respectively in the Biofield Energy Treated test formulation (G4) group compared with the disease control (G2) group. On the other hand, the cellular immune response i.e. CD4+ and CD8+ counts were significantly increased by 56.21% and 73.6%, respectively in the G4 group compared with the G2. Hematology analysis showed an increased level of TLC and lymphocytes by 14.85% and 21.42%, respectively in the G4 group compared to the G2. Lipid profile analysis showed a significant decrease in the level of total cholesterol, triglycerides, LDL, and VLDL in the G4 group compared with the G2. Further, results suggests a significant decrease in the hepatic biomarkers such as SGOT, SGPT, and cardiac enzyme, CK-MB by 18.54%, 19.12%, and 26.23%, respectively in G4 group compared to the G2 group. However, the testosterone level was significantly decreased in G4 group and untreated test formulation (G5) groups by 53.87% and 26.25%, respectively compared with the G2 group. Additionally, the antioxidant assay data showed an altered level of tested enzymes such as LPO, SOD and CAT, in the G4 group compared with the G2 group. Therefore, it can be concluded that the Biofield Energy Treated test formulation showed a significant improved cellular and humoral immunity, hematological and biochemical profile as compared with the untreated test formulation. As a result, it can be established that The Trivedi Effect®-Biofield Energy Healing has the significant capacity for immunomodulatory effect, which may also be useful in organ transplants, anti-aging, and stress management by improving overall health and quality of life.

Conclusion

In conclusion, the Biofield Energy Treated herbomineral formulation exhibited significant immunomodulatory action after oral administration in male Sprague Dawley rats. The results of humoral immune biomarkers showed a significant alterations in the level of IgG and IgM in the Biofield Energy Treated test formulation group compared with the disease control group (G2). In addition, the results of cellular immune responses i.e. CD4+ and CD8+ were significant improved compared with the disease control group. The significant increase in the percentage of CD4+ and CD8+ level was found by 56.21% and 73.6%, respectively in the Biofield Energy Treated test formulation (G4) compared with the G2 group. However, the improved cellular immune response was highly significant i.e. CD4+ and CD8+ levels by 9.07% and 14.17%, respectively in the Biofield Energy Treated test formulation compared with the untreated test formulation (G5). The hematology parameters such as the level of TLC and lymphocytes were increased by 14.85% and 21.42%, respectively in the Biofield Energy Treated test formulation group (G4) compared with the disease control group (G2). Biochemical analysis showed that the glucose concentration was altered (11.44%), while levels of TC, triglycerides, LDL, and VLDL were decreased in the Biofield Energy Treated test formulation (G4) group compared with the disease control (G2) group. Further, the analysis of hepatic and cardiac biomarkers in serum sample showed a significant decrease in the level of SGOT, SGPT, and CK-MB by 18.54%, 19.12%, and 26.23%, respectively in the Biofield Energy Treated test formulation (G4) compared with the disease control (G2) group. However, serum testosterone level was significantly decreased by 53.87% in G4 group, while untreated test formulation group showed decreased level by 26.25% as compared with the diseases control (G2) group. Further, the antioxidant profile showed a significant alteration in the level of LPO, SOD, and CAT in the G4 group compared with the G2 group.

Overall, the current experimental findings suggested the Trivedi Effect®-Biofield Energy Healing Treatment done remotely by the twenty Biofield Energy Healers enhanced the herbomineral test formulation’s anti-inflammatory and immunomodulatory properties that can be used to improve the overall health. Thus, the Biofield Energy Treated test formulation may act as an effective anti-inflammatory and immunomodulatory product, and it can be used as a CAM with a safe therapeutic index for various autoimmune disorders such as Lupus, Systemic Lupus Erythematosus, Fibromyalgia, Addison Disease, Hashimoto Thyroiditis, Celiac Disease (gluten-sensitive enteropathy), Multiple Sclerosis, Dermatomyositis, Graves’ Disease, Myasthenia Gravis, Pernicious Anemia, Aplastic Anemia, Scleroderma, Psoriasis, Rheumatoid Arthritis, Reactive Arthritis, Type 1 Diabetes, Sjogren Syndrome, Crohn’s Disease, Vasculitis, Vitiligo, Chronic Fatigue Syndrome and Alopecia Areata, as well as inflammatory disorders such as Irritable Bowel Syndrome (IBS), Asthma, Ulcerative Colitis, Alzheimer’s Disease, Parkinson’s Disease, Atherosclerosis, Dermatitis, Hepatitis, and Diverticulitis. Further, the Biofield Energy Healing Treated test formulation can also be used in the prevention of immune-mediated tissue damage in cases of organ transplants, for anti-aging, stress prevention and management, and in the improvement of overall health and quality of life.