In Vitro Effect of Biofield Energy Treated DMEM On Mitochondrial Biogenesis Using Myoblasts Cell Line, C2C12

Journal: Journal of Pharmaceutical and Pharmacological Sciences PDF  Web

Published: 16 April, 2019 Volume: 2019 Issue: 01 Pages: 1-5

DOI: 10.29011/2574-7711.100077 ISSN: 2474-7711

Authors: Alice Branton, Snehasis Jana

Citation: Branton A, Jana S (2019) In Vitro Effect of Biofield Energy Treated DMEM On Mitochondrial Biogenesis Using Myoblasts Cell Line, C2C12. J Pharma Pharma Sci: JPPS-177. DOI: 10.29011/2574-7711.100077

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Abstract

Mitochondria, a signaling organelles are responsible for energy metabolism within the cell, while its dysfunction in any form could results in the cell death due to an increased oxidative stress and that leads to various metabolic diseases. The aim of the present study was to examine the effect of Consciousness Energy Healing based DMEM medium on murine myoblasts (C2C12) cells to evaluate the mitochondrial mass content using 10-N-nonyl acridine orange (NAO) dye assay. The test item, DMEM was divided into three parts. First part did not receive any sort of treatment and defined as the untreated DMEM group. The second and third parts were treated with the one-time and two-times Biofield Energy Treatment by a renowned Biofield Energy Healer, Alice Branton and coded as the one-time Biofield Energy Treated DMEM (BT-I) and two-times Biofield Energy Treated DMEM (BT-II) groups, respectively. Cell viability of the test items using MTT assay showed 72.32%, 97.89%, and 104.96% viable cells in the untreated DMEM, BT-I, and BT-II groups, respectively suggested that the test items were nontoxic and safe in murine myoblasts (C2C12) cells. Further, the mitochondrial mass content in terms of Fluorescence Unit (FU) was significantly (p≤0.05) increased by 43.11% and 64.52% in the BT-I and BT-II groups, respectively in C2C12 cells compared to the untreated DMEM group. Thus, overall experimental data suggested that two-times blessed DMEM showed a significant improvement of mitochondrial mass content and results in better thermogenesis. In the present study, results demonstrated that an increased mitochondrial mass content in the cells, when treated with The Trivedi Effect®. This indicates that the Biofield Energy Treated DMEM has the great potential to improve thermogenesis, which can be used against various metabolic diseases, such as insulin resistance, type-2 diabetes, and cardiovascular diseases.

Conclusion

MTT assay of the test item (DMEM) in C2C12 cells showed a significant improved cell viability with the ranges from 97.89% to 104.96% in different test item groups. Besides, the mitochondrial mass content was significantly (p≤0.05) increased by 43.11% and 64.52% in the one-time Biofield Energy Treated DMEM (BT-I) and two-times Biofield Energy Treated DMEM (BT-II) groups, respectively in C2C12 cells compared to the untreated DMEM group. Thus, Biofield Energy Healing based DMEM can be significantly used to improve the energy level, endurance, body energy, which can be utilized against many diseases such aging, diabetes, cancer, depression, hypertension, cardiovascular disease, aging, and physical strength. The Biofield Energy Treated (The Trivedi Effect®) DMEM were found to have a significant impact on thermogenesis, which might significantly improve the mitochondrial content in muscle cells. Therefore, the Consciousness Energy Healing based DMEM might be a suitable alternative media for cell growth. It can be useful for the management of various disorders such as Lupus, Systemic Lupus Erythematosus, Fibromyalgia, Addison Disease, Hashimoto Thyroiditis, Celiac Disease (gluten-sensitive enteropathy), Multiple Sclerosis, Dermatomyositis, Graves’ Disease, Myasthenia Gravis, Pernicious Anemia, Aplastic Anemia, Scleroderma, Psoriasis, Rheumatoid Arthritis, Reactive Arthritis, Type 1 Diabetes, Sjogren Syndrome, Crohn’s Disease, Vasculitis, Vitiligo, Chronic Fatigue Syndrome and Alopecia Areata, as well as inflammatory disorders such as Irritable Bowel Syndrome (IBS), Asthma, Ulcerative Colitis, Alzheimer’s Disease, Parkinson’s Disease, Atherosclerosis, Dermatitis, Hepatitis, and Diverticulitis. Further, the Biofield Energy Healing Treated test formulation can also be used in the prevention of immune-mediated tissue damage in cases of organ transplants (for example heart transplants, kidney transplants and liver transplants), for anti-aging, stress prevention and management, and in the improvement of overall health and Quality of Life.