In Vitro Cell-Based Biomarkers Study of Vital Organs: Impact of the Biofield Energy Based Test Formulation

Journal: Journal of Human Health Research PDF  Web

Published: July 18, 2019 Volume: 1 Issue: 2 Pages: 22-38

DOI: 10.14302/issn. 2576-9383.jhhr-19-2945 ISSN: 2576-9383

Authors: Krista Joanne Callas, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana

Citation: Krista Joanne Callas, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak et al. (2019) In Vitro Cell-Based Biomarkers Study of Vital Organs: Impact of the Biofield Energy Based Test Formulation. Journal of Human Health Research - 1(2):22-37.

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Abstract

The present study was undertaken to evaluate the impact of Biofield Energy Treated test formulation using multiple cell-lines. The test formulation and cell media (Med) was divided into two parts; one part was untreated (UT) and other part received Biofield Energy Treatment remotely by a renowned Biofield Energy Healer, Krista Joanne Callas, USA and labeled as Biofield Energy Treated (BT) test item (TI)/Med. Based on cell viability, test formulation was found safe. Cytoprotective action of test formulation showed significant restoration of cell viability by 89.9% and 106.4% in human cardiac fibroblasts cells (HCF) cells, while improved restoration of cell viability by 77.3% and 69% in HepG2 cells compared to untreated. Cellular restoration in A549 cells was also improved by 141.2% and 157.1% compared to untreated. ALP activity was significantly increased by 118.7% and 140.7% in UT-Med + BT-TI and BT-Med + UT-TI, respectively at 0.1 µg/mL than untreated. Percent cellular protection of HCF (heart) cells (decreased of LDH activity) was significantly increased by 89.9% and 106.4% in UT-Med + BT-TI and BT-Med + BT-TI, respectively than untreated. HepG2 cells protection (decreased ALT activity) was increased by 59.8% in BT-Med + BT-TI than untreated. Superoxide dismutase (SOD) level was increased by 22.8% in BT-Med + BT-TI than untreated. Serotonin level was significantly increased by 361.7% and 197.6% in BT-Med + UT-TI and BT-Med + BT-TI, respectively than untreated in human neuroblastoma cells (SH-SY5Y). However, relative quantification (RQ) of vitamin D receptor (VDR) was significantly increased by 116.5%, 214.7%, and 241.5% in UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI, respectively than untreated in MG-63 cells. Overall, data showed a significant improvement of organ-specific functional enzyme biomarkers. Thus, Biofield Energy Treated Test formulation (the Trivedi Effect®) would be useful for multiple organs health that can be beneficial against coronary artery disease, arrhythmias, congenital heart disease, cardiomyopathy, cirrhosis, liver cancer, hemochromatosis, asthma, chronic bronchitis, cystic fibrosis, osteoporosis, etc.

Conclusion

The safe concentrations of the test formulation was analyzed using MTT assay and was found as safe and non-toxic against all the tested cell lines. Cytoprotective activity against t-BHP induced cell damage using human cardiac fibroblasts cells (HCF) showed 89.9% (63.75 µg/mL) and 106.4% (63.75 µg/mL) restoration of cell viability in UT-Med + BT-TI and BT-Med + BT-TI groups, respectively as compared to untreated. Moreover, in HepG2 cells restoration of cell viability was observed as 77.3% and 69% in BT-Med + UT-TI and BT-Med + BT-TI groups, respectively as compared to untreated at 10 µg/mL. In A549 cells, cellular restoration was improved by 141.2% and 157.1% at 0.1 µg/mL in UT-Med + BT-TI and BT-Med + BT-TI groups, respectively as compared to untreated. ALP activity in Ishikawa cells showed significantly increased by 118.7% and 140.7% in UT-Med + BT-TI and BT-Med + UT-TI groups, respectively at 0.1 µg/mL as compared to untreated. Similarly, ALP activity in MG-63 cells was reported by 69.4%, 71.8%, and 71.4% cellular protection in UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI groups, respectively at 50 µg/mL compared with the untreated. The percent protection of HCF cells (decreased of LDH activity) was significantly increased by 89.9% (63.75 µg/mL) and 106.4% (63.75 µg/mL) in UT-Med + BT-TI and BT-Med + BT-TI groups, respectively as compared to untreated. The percent protection of HepG2 cells (decreased of ALT activity) was significantly increased by 39.2% (1 µg/mL), 37.3% (25.5 µg/mL), and 59.8% (10 µg/mL) in UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI groups, respectively as compared with the untreated. The percent protection of A549 (lungs) cells (increased of SOD activity) was significantly increased by 22.1% at 10 µg/mL in BT-Med + UT-TI group, while 22.8% and 22.1% at 1 and 10 µg/mL, respectively in BT-Med + BT-TI as compared to untreated. Serotonin level was significantly increased in SH-SY5Y cells by 361.7% (10 µg/mL) and 197.6% (25 µg/mL) in BT-Med + UT-TI and BT-Med + BT-TI groups, respectively compared with the untreated. The relative quantification (RQ) of vitamin D receptors (VDRs) level was significantly increased by 197%, 214.7%, and 198.8% at 1, 10, and 50 µg/mL, respectively in BT-Med + UT-TI group; while, 241.5%, 221%, and 235.8% at 1, 10, and 50 µg/mL, respectively in BT-Med + BT-TI as compared to untreated. Therefore, this study concluded that Biofield Energy based test formulation significantly improved the overall functioning of heart, liver, bones, neuronal, and lungs parameters against t-BHP induced oxidative stress. Thus, the Biofield Energy Treatment (The Trivedi Effect®) can be used for the prevention of various types of cardiac disorders such as stroke, congestive heart failure, congenital heart disease, rheumatic heart disease, valvular heart disease, venous thrombosis, etc. Besides, it would also protect against many hepatic disorders (cirrhosis, liver cancer, hemochromatosis, and Wilson disease), lungs disorders (asthma, chronic bronchitis, emphysema, cystic fibrosis, and pneumonia), and many immune disorders. In addition, this novel test formulation can also be utilized for organ transplants (i.e., kidney, liver, and heart transplants), hormonal imbalance, aging, and various inflammatory and immune-related disease conditions like Asthma, Graves’ Disease, Dermatitis, Diabetes, Parkinson’s disease, Myasthenia Gravis, Ulcerative Colitis (UC), Atherosclerosis, etc. to improve overall health and Quality of Life.