In Vitro Assessment of Estrogenic Potential of Biofield Energy Treatment using Human Endometrial Adenocarcinoma Cell Line


Published: Dec 03, 2018 Volume: 2 Issue: 2 Pages: 12-19

DOI: 10.14302/issn.2381-862X.jwrh-18-2459 ISSN: 2381-862X

Authors: Mahendra Kumar Trivedi, Snehasis Jana

Citation: Mahendra Kumar Trivedi, Snehasis Jana (2018) In Vitro Assessment of Estrogenic Potential of Biofield Energy Treatment using Human Endometrial Adenocarcinoma Cell Line. Journal of Woman's Reproductive Health - 2(2):12-19.



The objective of the study was to investigate the effect of Consciousness Energy Healing based DMEM medium on the level of alkaline phosphatase enzyme (ALP) activity in Ishikawa cells. The test item, DMEM medium was divided into two parts. One part of the test item received Consciousness Energy Healing Treatment by a renowned Biofield Energy Healer, Mahendra Kumar Trivedi and was labeled as the Biofield Energy Treated DMEM, while the other part did not receive any treatment, and defined as the untreated DMEM group. The cell viability using MTT assay of the Biofield Energy Treated DMEM group was observed as 108%, which indicated that the test item was safe and non-toxic. The estrogenic potential using ALP level showed a significantly increase by 73.21% in the Biofield Energy Treated DMEM group as compared to the untreated DMEM group. Overall, the experimental data suggested that the Biofield Energy Treated DMEM has significantly improved ALP level, which play a vital role for the promotion and maintenance of estrogen level. Based on the study outcomes, it is concluded that Biofield Energy Healing Treatment showed a significant improved ALP level, which can be used in various estrogenic disorders such as hypophosphatasia, osteoporosis, severe anemia, malnutrition, hypothyroidism, magnesium deficiency, heart surgery, aplastic anemia, chronic myelogenous leukemia, enteritis in children, Wilson’s disease, pernicious anemia, bacterial infection and intrauterine infection is a leading cause of pelvic inflammatory disease, subfertility, infertility, endometritis, early pregnancy loss, fetal defects, and preterm birth.


Biofield Energy Treatment (The Trivedi Effect®) on DMEM was studied to evaluate the estrogenic potential and in vitro results showed a significant improvement with respect to ALP activity. Cell viability data using MTT assay showed a significant improved cell viability after Biofield Energy Healing Treatment with 108% in the Biofield Energy Treated group, while more than 75% cell viability was observed in the positive control group reflecting that the test sample was found as safe and nontoxic. Further, the level of ALP was significantly increased by 73.21% in the Biofield Energy Treated DMEM group as compared with the untreated DMEM group. Thus, the Biofield Energy Treated (The Trivedi Effect®) DMEM were found to have a significant impact on ALP level, which results in a better estrogenic potential and osteoblastic differentiation. Therefore, the Consciousness Energy Healing based DMEM might be a suitable alternative media for cell growth. It can be useful for the management of various estrogenic and menstrual disorders viz. Dysmenorrhea with painful cramps, Premenstrual Syndrome (PMS), Menorrhagia, Oligomenorrhea, Amenorrhea, and Missed periods. Thus, Biofield Energy Treatment would be useful to control the estrogen balance and thus control overall hormonal balance, which can be useful against stress, aging, osteoporosis, various bone diseases, cell differentiation, could improve cell-to-cell communication, normal cell growth, neurotransmission, cell cycling and proliferation, hormonal balance, skin health, immune and cardiovascular functions. Besides, it control the hormonal imbalance, various immune related disease conditions such as Hashimoto Thyroiditis, Aplastic Anemia, Hepatitis, Diverticulitis, Pernicious Anemia, Sjogren Syndrome, Myasthenia Gravis, Parkinson’s Disease, Asthma, Graves’ Disease, Dermatomyositis, Multiple Sclerosis, Ulcerative Colitis, Alzheimer’s Disease, Dermatitis, Irritable Bowel Syndrome, Diabetes, Atherosclerosis, Systemic Lupus Erythematosus, stress, etc. with a safe therapeutic index to improve overall health, and Quality of Life.