Improved Overall Bone Health Activity in MG-63 Cell Line after Biofield Energy Healing Treatment on Vitamin D3.

Journal: Global Journal of Environmental Science and Technology PDF  

Published: 22 June, 2018 Volume: 6 Issue: 2 Pages: 529-538

ISSN: 2360-7955

Authors: Kathleen Starr Vagt, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana

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Abstract

The aim of the study is to evaluate the role of The Trivedi Effect®-Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health parameters in vitro using MG-63 cells. The parameters of bone health tested are alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization. The test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Kathleen Starr Vagt and samples were defined as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay exhibited increased cell viability more than 72% with safe and nontoxic profile among test samples on MG-63 cell line. ALP was significantly increased by 140.8% (50 µg/mL), 167.3% (100 µg/mL),and 223.5% (50 µg/mL) in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared with the untreated group. The level of collagen was significantly increased by 120.7% and 93% at 1 and 10 µg/mL, respectively in the UT-DMEM+BT-TI group, while 46.7%, 100.9%, and 29.2% at 1, 10, and 50 µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased collagen level by 60.9%, 93%, and 14.4% at 1, 10, and 50 µg/mL, respectively as compared with the untreated test item and DMEM group. The percent of bone mineralization was significantly increased by 95.9% at 100 µg/mL in the UT-DMEM+BT-TI group, while 162% and 35.5% at 50 and 100 µg/mL, respectively in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 57% and 107.4% at 50 and 100 µg/mL, respectively as compared with the untreated group.T hus, the study results concluded that The Trivedi Effect Treatment would be the best alternative treatment for the maintenance of strong and healthy bones and quality of life. Further, it regulates the osteoblast function and improved the level of collagen, ALP, and calcium absorption in wide range of bone disorders along with wide range of adverse bone health conditions.

Conclusion

The Consciousness Energy Healing based vitamin D3 showed a significant improved overall bone health. MTT assay data showed significant improved cell viability with more than 72% among different experimental groups. Bone health parameters such as ALP was increased by 140.8% and 15.2% at 50 and 100 µg/mL, respectively in the UT-DMEM+BT-TI, while 167.3% at 100 µg/mL in the BT-DMEM+UT-TI group as compared with the untreated test item and DMEM group. In addition, BT-DMEM+BT-TI group showed an increased ALP level by 223.5% at 50 µg/mL. The level of collagen was significantly increased by 120.7%, 93%, and 14.4% at 1, 10, and 50 µg/mL, respectively in the UT-DMEM+BT-TI, while46.7%, 100.9%, and 29.2% at 1, 10, and 50 µg/mL, respectively in the BT-DMEM+UT-TI group. The level of collagen was increased by 60.9%, 93%, and 14.4% at 1, 10, and 50 µg/mL, respectively in BT-DMEM+BT-TI group as compared with the untreated test item and DMEM group. Similarly, the bone mineralization percent was significantly increased by95.9% at 100 µg/mL in the UT-DMEM+BT-TI group, while 19.7%, 162%, and 35.5% at 10, 50, and 100 µg/mL, respectively in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 57% and 107.4% at 50 and 100 µg/mL, respectively as compared with the untreated group. The Bone health parameters were significantly improved among the Biofield Energy Treated vitamin D3 test samples in MG-63 cells. Overall, the Biofield Energy Treated (The Trivedi Effect®) test samples were found to have a significant impact on tested bone health parameters viz. collagen, bone mineralization, and ALP, which are very vital to combat the bone disorders. Therefore, the Consciousness Energy Healing based vitamin D3 might be a suitable alternative nutritional supplement, which could be useful for the management of various bone related disorders viz. osteoporosis, Paget’s disease of bone, rickets, deformed bones, osteomalacia, bone and/or joint pain, increased frequency of fractures, osteoma, hormonal imbalance, stress, aging, bone loss and fractures, and other bone diseases that are caused by poor nutrition, genetics, or problems with the rate of bone growth or rebuilding. Biofield Energy Treated Vitamin D3 can be useful as anti-inflammatory, anti-aging, anti-stress, anti-arthritic, anti-osteoporosis, anti-cancer, anti-apoptotic, wound healing, anti-psychotic and anti-fibrotic roles. It also influence cell-to-cell communication, normal cell growth, cell differentiation, neurotransmission, cell cycling and proliferation, hormonal balance, skin health, immune and cardiovascular functions. Besides, it can also be utilized in hormonal imbalance, aging, and various immune related disease conditions such as Multiple Sclerosis, Alzheimer’s Disease, Dermatitis, Atherosclerosis, stress, Irritable Bowel Syndrome, Systemic Lupus Erythematosus, Pernicious Anemia, Aplastic Anemia, Hepatitis, Diverticulitis, Graves’ Disease, Dermatomyositis, Asthma, Hashimoto Thyroiditis,Diabetes, Myasthenia Gravis, Ulcerative Colitis, Sjogren Syndrome, Parkinson’s Disease, etc. with a safe therapeutic index to improve overall health, and quality of life.