Journal: International Journal of Immunology PDF
Published: 2016/12/08 Volume: 4 Issue: 5 Pages: 35-45
DOI: 10.11648/j.iji.20160405.12 ISSN: 2329-177X (Print) 2329-1753 (Online)
Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Aileen Carol Lee, Aksana Hancharuk, Carola Marina Sand, Debra Jane Schnitzer, Rudina Thanasi, Eileen Mary Meagher, Faith Ann Pyka, Gary Richard Gerber, Johanna Catharina Stromsnas, Judith Marian Shapiro, Laura Nelson Streicher, Lorraine Marie Hachfeld, Matthew Charles Hornung, Patricia M. Rowe, Sally Jean Henderson, Sheila Maureen Benson, Shirley Theresa Holmlund, Stephen P. Salters, Mayank Gangwar, Snehasis Jana
Citation: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Aileen Carol Lee, Aksana Hancharuk, Carola Marina Sand, Debra Jane Schnitzer, Rudina Thanasi, Eileen Mary Meagher, Faith Ann Pyka, Gary Richard Gerber, Johanna Catharina Stromsnas, Judith Marian Shapiro, Laura Nelson Streicher, Lorraine Marie Hachfeld, Matthew Charles Hornung, Patricia M. Rowe, Sally Jean Henderson, Sheila Maureen Benson, Shirley Theresa Holmlund, Stephen P. Salters, Mayank Gangwar, Snehasis Jana. Impact of Biofield Energy Treated Herbomineral Formulation (The Trivedi Effect®) on Mouse Dendritic and Splenocyte Cells for Modulation of Pro-inflammatory Cytokines. International Journal of Immunology. Vol. 4, No. 5, 2016, pp. 35-45. doi: 10.11648/j.iji.20160405.12
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The use of herbomineral formulation in the healthcare sector for different chronic diseases is gaining popularity due to its fewer side effects, high safety profile, and cost effectiveness. A new proprietary herbomineral formulation was formulated, consisting of four essential ingredients viz. herbal root extract (ashwagandha), and minerals (zinc, magnesium, and selenium). The study aims to evaluate the in vitro effect of Biofield Energy Healing (The Trivedi Effect®) on the test formulation using murine dendritic (DCs) and splenocyte cells. The herbomineral formulation was divided into two parts; one was represented as control, while the other part was treated with the Biofield Energy Healing Treatment remotely by eighteen renowned Biofield Energy Healers (The Trivedi Effect®) and defined as the Biofield Treated formulation. The effect of the test formulation on these cells were monitored by an estimation of pro-inflammatory cytokines level such as tumor necrosis factor (TNF-α), macrophage inflammatory protein (MIP-1α), and interleukin (IL-1β) in cell culture supernatants at the non-cytotoxic concentrations of the test formulation using MTT assay. The DCs were treated with the Biofield Energy Treated test formulation at different concentrations (i.e. 1.05 to 1052.5 µg/mL) for 24 hours, and the results showed significant (p≤0.001) suppression of TNF-α levels at all the tested concentrations with a maximum percentage decrease by 43.64% at 5.2 µg/mL concentration in the Biofield Treated formulation as compared with the untreated test formulation. Further, the Biofield Treated formulation also demonstrated inhibition of MIP-1α and IL-1β at a concentration range of 0.0000105 to 10.5 µg/mL in LPS stimulated splenocyte cells. There was a significant (p≤0.001) inhibition of MIP-1α (26.52%) and IL-1β (35.28%) in the Biofield Treated test formulation at concentration 0.000105 µg/mL and 0.0000105 µg/mL, respectively in comparison to the untreated test formulation. Overall, these results suggest that the Biofield Energy Healing based herbomineral formulation (The Trivedi Effect®) significantly down-regulated the tested cytokines expression in DCs and splenocyte cells as compared to the untreated formulation. Therefore, the Biofield Healing based formulation might be useful as a better anti-inflammatory product for many chronic and acute inflammatory disease conditions and autoimmune disorders. The Biofield Energy Treatment based formulation can also be effectively applied in cases of organ transplants, stress management and anti-aging by improving overall health.
Based on these results, it was concluded that the Biofield Energy Treated formulation modulates the DCs and splenocyte cells function. Further, the Biofield Treated formulation significantly inhibited the activity of pro-inflammatory cytokines such as TNF-α, MIP-1α, and IL-1β in both the tested immune cells as compared to the untreated test formulation. The results of MTT assay in DCs after 24 hours of treatment incubation with different concentrations of the Biofield Treated formulation suggest a safe concentration range of 1.05 to 105.2 µg/mL, which was tested for estimation of TNF-α. Similarly, MTT assay in splenocyte cells after 48 hours of treatment incubation with the different concentrations of the Biofield Treated formulation in the presence of 0.5 μg/mL LPS, suggest that the concentration range of 0.0000105 to 1.05 µg/mL was found safe with more than 80% cell viability, which were selected for MIP-1α and IL-1β estimation. The Biofield Treated formulation showed significant (p≤0.001) suppression of TNF-α level in DCs cells at all the tested concentration ranges (i.e. 1.05 to 1052.5 µg/mL), while maximum suppression was reported at 5.2 µg/mL by 43.64% as compared with the untreated test formulation. However, the levels of MIP-1α and IL-1β at the tested concentration range (0.0000105 to 10.5 µg/mL) in LPS stimulated splenocyte cells were significantly inhibited. Further, data suggests that inhibition of MIP-1α (26.52%) and IL-1β (35.28%) in the Biofield Treated formulation at 0.000105 and 0.0000105 µg/mL, respectively as compared with the untreated test formulation.
Overall, the inhibition of pro-inflammatory cytokines might prevent the over activation of the human immune system. Thus, it may strongly counteract the pro-inflammatory responses, which might prevent immune-mediated tissue damage. Through insight into immunomodulatory cytokines, the Biofield Energy Treated test formulation can provide the innovative strategy to affect the immune system, which could help combat various acute and chronic inflamatory diseases as a better complementary and alternative medicine. So, it can be concluded that The Trivedi Effect®-Biofield Energy Healing (TEBEH) has the capacity to modulate the immunoinflammatory activity of herbomineral formulations with a safe therapeutic index and a faster absorption rate that can enhance the human well-being in the biomedical health care system. This can be ulitized against many acute and chronic inflammatory diseases like Asthma, Chronic peptic ulcer, Tuberculosis, Hepatitis, Irritable Bowel Syndrome, Parkinson’s Disease, Rheumatoid arthritis, Chronic periodontitis, Ulcerative colitis, Crohn's disease, Chronic sinusitis, Systemic Lupus Erythematosus, Type 1 Diabetes and Chronic active hepatitis. Furthermore, the Biofield Energy Treatment Therapy applied to the herbomineral formulation can also be utilized for various autoimmune disorders such as Graves’ Disease, Lupus, Celiac Disease (gluten-sensitive enteropathy), Dermatomyositis, Hashimoto Thyroiditis, Addison Disease, Multiple Sclerosis (MS), Myasthenia Gravis, Aplastic Anemia, Reactive Arthritis, Sjogren Syndrome, Alopecia Areata, Fibromyalgia, Vitiligo, Pernicious Anemia, Psoriasis, Scleroderma, Chronic Fatigue Syndrome and Vasculitis, as well as to prevent the immune-mediated tissue damage in cases of organ transplants (for example kidney transplants, liver transplants and heart transplants) because of its safe therapeutic index and potential to modulate the immune system and improve overall health and quality of life. Overall, the Biofield Energy Healing Treated test formulation can also be used for anti-aging, stress prevention and management, and in the improvement of overall health and quality of life.