Immunomodulatory properties and biomarkers characterization of novel Withania somnifera based formulation supplemented with minerals in Sprague Dawley rats

Journal: Oriental Pharmacy and Experimental Medicine  Web

Published: 06.12.2016 Volume: 17 Issue: 1 Pages: 59-69

DOI: 10.1007/s13596-016-0255-5 ISSN: 1598-2386 (Print) 2211-1069 (Online)

Authors: Mahendra Kumar Trivedi, Mayank Gangwar, Sambhu Charan Mondal, Snehasis Jana

Citation: Trivedi, M.K., Gangwar, M., Mondal, S.C. et al. Orient Pharm Exp Med (2017) 17: 59. https://doi.org/10.1007/s13596-016-0255-5

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Abstract

Herbomineral formulations have been accepted world-wide due to its significant therapeutic outcomes. The study aimed to evaluate the immunomodulatory potential of novel herbomineral formulation (TEBEH) on Sprague Dawley rats using cyclophosphamide-induced immunosuppression. Major immune related parameters were monitored such haemagglutination assay, delayed type hypersensitivity reaction, cellular immune response (CD4+ and CD8+), IgG and IgM, hematological analysis, antioxidant profile, and estimation of major lipid biomarkers. The study results suggest herbomineral formulation significantly (p ≤ 0.05) improved the concentration of primary and secondary titre by 41.18 and 68%. However, the rat paw edema at 24 h showed increased edema by 77.78% (p ≤ 0.01). Herbomineral formulation exhibited increase in the CD4+ and CD8+ counts in animals by 28.03 and 60.70%, respectively. In addition, the concentrations of IgM and IgG were improved by 14.29 and 8.26% respectively. Besides, other supportive parameter such as hematology and antioxidant profile was significantly (p ≤ 0.001) improved, while free radical, lipid peroxidation was decreased. Moreover, the major lipid biomarkers were improved such as decreased triglycerides, LDL and VLDL by 15.88, 3.65, and 16.01%, respectively. Overall, the promising results of this study indicated that the herbomineral formulation, TEBEH can be employed to improve the cellular and humoral immunity along with improved hematological, antioxidant, and lipid profile.