Immunomodulatory Effect of the Biofield Energy Treated Cell Growth Medium (DMEM) and FBS in Immune Cells (Murine Macrophage RAW 264.7)

Journal: Journal of Cellular Immunology and Serum Biology PDF  Web

Published: December 27, 2018 Volume: 3 Issue: 2 Pages: 1-4

DOI: 10.15436/2471-5891.18.1968 ISSN: 2471-5891

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal and Snehasis Jana

Citation: Trivedi, MK., et al. Immunomodulatory Effect of the Biofield Energy Treated Cell Growth Medium (DMEM) and FBS in Immune Cells (Murine Macrophage RAW 264.7). (2018) J Cell Immunol Serum Biol 3(2): 1- 5.

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Abstract

The objective of the present study was to evaluate the immunomodulatory effect of Biofield Energy Treatment (The Trivedi Effect®) on Dulbecco’s Modified Eagle’s Medium (DMEM) and fetal bovine serum (FBS) in murine macrophage cells (RAW 264.7). The study parameters were evaluated using cell viability by MTT assay and estimation of proinflammatory cytokine like tumor necrosis factor - alpha (TNF-α) on immunomodulation using enzyme linked immune sorbent assay (ELISA). The cell viability using MTT assay data showed that more than 80% cell viability was observed in all the tested groups compared to the baseline control group (G1). The characteristics of cell morphology in the Biofield Energy Treated DMEM without FBS (G6) group showed some changes in the cell morphology as evidenced a transition from spherical to elongate and also showed maturation compared to the G1 group. The level of TNF-α expression was significantly reduced by 23.15% in the G3 group compared to the G1 group. Moreover, the secretion of TNF-α was altered by 38.06%, 35.77%, and 282.49% in the G4, G5, and G6 groups, respectively compared to the G1 group. The overall results demonstrated that the Trivedi Effect® - Consciousness Energy Healing Treatment has an impact on DMEM and FBS by alteration of cell morphology and reducing the level of TNF-α expression in immune cell (RAW 264.7). Therefore, the Trivedi Effect® treated DMEM and FBS might be useful as an immunomodulator for various immune-related disorders like Graves’ disease, rheumatoid arthritis, multiple sclerosis, etc.

Conclusion

The present study was conducted to evaluate the effect of Biofield Energy Treatment (The Trivedi Effect®) on DMEM and FBS for the assessment of TNF-α secretion in macrophage cells (RAW264.7). All the treatment groups demonstrated no adverse effects on cell viability with relation to the baseline control group (G1). However, all the tested groups showed >80% cell viability compared to the baseline control group (G1). Besides, the Biofield Energy Treated DMEM without FBS group (G6) showed some changes in the cell morphology as evidenced a transition from spherical to elongate and also showed maturation of cells compared to the G1 group. The level of TNF-α expression was significantly reduced by 23.15% in the Biofield Energy Treated FBS group (G3) with respect to the G1 group. In conclusion, The Trivedi Effect® - Consciousness Energy Healing Treatment might impact on DMEM and FBS by alteration of cell morphology and reducing the level of TNF-α expression in immune cell (macrophage RAW 264.7). Therefore, it can be useful as an immunomodulator for various immune-related disorders viz. multiple sclerosis, lupus, vasculitis, scleroderma, various types of hemolytic anemia, diabetes (type-1), graves’ disease, rheumatoid arthritis, etc. in the near future.Acknowledgements