Hepatoprotective Effect of Biofield Energy Treatment On Tert-Butyl Hydro Peroxide Induced Liver Injury in Hepatocellular Carcinoma Cell Line (HepG2)

Journal: Biomarkers & Applications  Web

Published: 25 December, 2018 Volume: 2018 Issue: 3 Pages: 1-7

DOI: 10.29011/2576-9588. 100029 ISSN: 2576-9588

Authors: Mahendra Kumar Trivedi and Snehasis Jana

Citation: Trivedi MK, Jana S (2018) Hepatoprotective Effect of Biofield Energy Treatment On Tert-Butyl Hydro Peroxide Induced Liver Injury in Hepatocellular Carcinoma Cell Line (HepG2). Biomark Applic BMAP-129. DOI: 10.29011/2576-9588. 100029

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Abstract

The present study was performed to investigate the Hepatoprotective potential of the Biofield Energy (The Trivedi Effect®) Treated test item, Dulbecco’s Modified Eagle Medium (DMEM) in HepG2 cells. The test item was distributed into two parts. One part received Consciousness Energy Healing Treatment by a renowned Biofield Energy Healer, Mahendra Kumar Trivedi and was labeled as the Biofield Energy Treated DMEM group and the other part referred as the untreated DMEM group, where no Biofield Treatment was provided. Results showed that more than 97% cell viability of the test items were observed by MTT assay, which indicated a safe and nontoxic nature of the test items. The Biofield Treated DMEM showed significant (p≤0.001) protection of cells by 10% against oxidative stress induced by t-BHP, while the untreated DMEM group showed 0.4% protection. The level of interleukin-8 (IL-8) was significantly (p≤0.01) reduced by 31.57% in Biofield Treated DMEM than untreated DMEM. The level of ALT enzyme activity was significantly (p≤0.001) reduced by 66% in Biofield Treated DMEM compared to untreated DMEM. Cholesterol level was significantly (p≤0.001) reduced by 46.23% in Biofield Treated DMEM than untreated DMEM. Besides, Biofield Treated DMEM group showed 51.18% increased the level of albumin compared to untreated DMEM group. Overall, results demonstrated that Biofield Treatment significantly protect the liver hepatocytes against oxidative stress. Therefore, Consciousness Energy Healing (The Trivedi Effect®) Treatment might be useful as a hepatoprotectant against different types of liver injuries like cirrhosis, alcohol abuse, hemochromatosis, Wilson’s disease, Gilbert’s disease, cholangiocarcinoma, steatosis, Budd-Chiari syndrome, etc.

Conclusion

In summary, the study results Showed That the Test Items (DMEM) were found as safe and non-toxic on the basis of MTT cell viability assay. The Biofield Energy Treated test item (DMEM) showed a significant (p≤0.001) protection of cells by 10% from the oxidative damage induced by t-BHP, while the untreated DMEM group showed 0.4% protection. IL-8 level was significantly (p≤0.01) reduced by 31.57% in the Biofield Energy Treated DMEM group compared to the untreated DMEM group. Moreover, ALT enzyme activity was significantly (p≤0.001) reduced by 66% in the Biofield Energy Treated DMEM group compared to the untreated DMEM group. Cholesterol level was significantly (p≤0.001) reduced by 46.23% in the Biofield Energy Treated DMEM group compared to the untreated DMEM group. Further, Biofield Energy Treated DMEM group showed 51.18% increased the level of albumin compared to the untreated DMEM group. It is therefore concluded that, The Trivedi Effect® - Consciousness Energy Healing Treatment significantly protect hepatocytes and could be used as an alternative treatment approach for the management of various types of hepatobiliary disorders viz. cirrhosis, acute hepatitis A, B, C, D, and E, cholestasis, chronic viral hepatitis, portal hypertension in schistosomiasis, necrosis, toxoplasmosis, hepatosplenic schistosomiasis, liver abscess, autoimmune hepatitis, granulomatous hepatitis, primary biliary cholangitis (primary biliary cirrhosis), phlebitis of the portal vein, etc. In broad perspective, it could be useful to improve cell-to-cell messaging, normal cell growth and differentiation, cell cycling and proliferation, neurotransmission, skin health, hormonal balance, immune and cardiovascular functions. Moreover, it can also be utilized in aging, and various inflammatory and immune-related disease conditions like Ulcerative Colitis (UC), Alzheimer’s Disease (AD), Dermatitis, Graves’ Disease, Asthma, Irritable Bowel Syndrome (IBS), Hashimoto Thyroiditis, Pernicious Anemia, Sjogren Syndrome, Diabetes, Multiple Sclerosis, Systemic Lupus Erythematosus (SLE), Aplastic Anemia, Hepatitis, Dermatomyositis, Parkinson’s Disease, Myasthenia Gravis, stress, etc.