Journal: Open Access Journal of Reproductive System and Sexual Disorders
Published: March 20, 2019 Volume: 2 Issue: 3 Pages: 206-210
DOI: 10.32474/OAJRSD.2019.02.000139 ISSN: 2641-1644
Authors: Dahryn Trivedi1 and Snehasis Jana
Citation: Dahryn T, Snehasis J. Evaluation of Estrogenic Activity of Biofield Energy Treatment for the Potential of Menstrual Disorders Using Ishikawa Cell Line. Open Acc J Repro & Sexual Disord 2(3)- 2019. OAJRSD.MS.ID.000139. DOI: 10.32474/OAJRSD.2019.02.000139.
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Alkaline phosphatase (ALP) plays a vital role in the endometrium development, maturation, endocrine function, and osteoblastic differentiation. The present investigation explained the role of Consciousness Energy Healing based test item (DMEM medium) on ALP in Ishikawa cells. After the division of the test item into two parts; first, part received Biofield Treatment by a renowned Biofield Energy Healer, Dahryn Trivedi and was labeled as the Biofield Treated DMEM, while the second part did not receive any treatment, and denoted as the untreated DMEM. MTT data showed 142.56% viable cells in the Biofield Treated test item, which indicated that the test item was found as safe and non-toxic. Besides, the estrogenic potential concerning ALP level was significantly (p≤0.001) increased by 65.18% in the Biofield Treated DMEM group than untreated DMEM. Thus, the experimental data suggested that the Biofield Energy Treated DMEM would play a significant role for the growth of Ishikawa cells along with an improved ALP level, which play a vital role in the promotion and maintenance of estrogen level and can be used against various estrogenic disorders viz. endometritis, hypophosphatasia, osteoporosis, infertility, severe anemia, malnutrition, fetal defects, early pregnancy loss, and preterm birth.
Cell viability data using MTT assay showed significantly improved cell viability after Biofield Energy Healing Treatment with 142.56% in the Biofield Energy Treated group, while up to 96% in the positive control group that showed the safety profile of the test samples. ALP was significantly increased by 65.18% in the Biofield Energy Treated DMEM group compared with the untreated DMEM group. Thus, Consciousness Energy Healing Treatment (The Trivedi Effect®) on DMEM was found to have a significant impact on the ALP level, which results in a better estrogenic potential and osteoblastic differentiation. Therefore, to the untreated DMEM, the Biofield Energy Treated DMEM would be highly significant in the growth and viability of Ishikawa cells. Thus, the Consciousness Energy Healing based DMEM might be a suitable alternative media for cell growth. The potential of Biofield Energy Treatment could be further extendable for the management of menstrual disorders viz. premenstrual syndrome, dysmenorrhea with painful cramps, oligomenorrhea, amenorrhea, and menorrhagia. Thus, Consciousness Energy Healing Treatment would be useful to regulate the estrogen balance, which can be useful against aging, stress, and immune-related disorders.