Published: April 10, 2019 Volume: 5 Issue: 1 Pages: 7-13
DOI: 10.15436/2381-0858.19.2451 ISSN: 2381-0858
Authors: Dahryn Trivedi, Snehasis Jana
Citation: Dahryn, T., et al. Evaluation of A Consciousness Energy Healing Based Test Formulation for Skin Health (2019) Invest Demerol and Venereol Res 5(1): 7-13.
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This study was investigated the effect of Consciousness Energy Healing (The Trivedi Effect®) based test formulation and DMEM medium on skin health in B16-F10, HaCaT, and HFF-1 cells. The test formulation and DMEM were assigned into two parts. First part was received the Biofield Treatment by a renowned Biofield Energy Healer, Dahryn Trivedi and were defined as the Biofield Treated samples (BT), while the second parts were denoted as the untreated test samples (UT). MTT assay data showed more than 76% cells were viable in all the tested concentrations in three different cell lines, indicating that the test formulation was safe and nontoxic. The BrdU assay showed the percent cell proliferation was increased by 15.88% in the UT-DMEM + BT- Test formulation compared to the UT-DMEM + UTTest formulation group. Elastin was increased significantly (p ≤ 0.001) by 64.37%, 29.46% and 38.75% in UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation, respectively at 100 μg / mL compared to the untreated test formulation. Hyaluronic acid was significantly increased by 17.74%, 96.20%, and 25.03% in UT-DMEM + BT- Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 10 μg / mL compared to untreated group. The level of melanin was significantly decreased by 8.60% (p ≤ 0.05), 12.68% (p ≤ 0.001), and 21.73% (p ≤ 0.001) in the UT-DMEM + BT- Test formulation group at 0.1, 1.0, and 10 μg / mL, respectively compared to the untreated group. Protection of skin cells after UV-B exposure data showed that the cell viability was significantly increased by 31.15% and 10.84% in the UT-DMEM + BTTest formulation and BT-DMEM + UT-Test formulation groups, respectively at 50 μg / mL compared to the untreated group. Wound healing data exhibited that the wound area was recovered with new cells by 84.91%, 92.31%, 35.09%, and 41.67% at 10, 50, 100, and 200 μg / mL in the BT-DMEM + BT- Test formulation group, respectively in HaCaT cells compared to the untreated group. The experimental data envisaged that the Biofield Energy Treated DMEM and test formulation showed significant skin protective activity. Thus, the Biofield Energy Treated test formulation could be useful for the development of effective cosmetic products for the nourishment of several skin problems such as fine lines, wrinkles, texture, pore size, elasticity, skin color / clarity, redness, hydration, to maintain the overall skin quality, etc.
The cell viability exhibited more than 76% cells were viable in all the tested concentrations and found as nontoxic. The cell proliferation (%) using BrdU assay was increased by 15.88% in the UT-DMEM + BT-Test formulation compared to the UTDMEM + UT-Test formulation group. Elastin was significantly increased by 38.75%, 64.37%, and 29.46% in the (BT-DMEM + BT-Test formulation), (UT-DMEM + BT-Test formulation), and (BT-DMEM + UT-Test formulation) groups, respectively at 100 μg / mL than untreated. Hyaluronic acid was significantly increased by 25.03%, 17.74%, and 96.20% in the BT-DMEM + BT-Test formulation, UT-DMEM + BT-Test formulation, and BT-DMEM + UT-Test formulation groups, respectively at 10 μg / mL compared to the untreated group. Melanin level was significantly reduced by 12.68% and 21.73% in the UT-DMEM + BTTest formulation group at 1 and 10 μg / mL, respectively with respect to the untreated group. Cellular protection with respect to UV-B, revealed that the level of cell viability was increased by 31.15% and 10.84% in the UT- DMEM + BT- Test formulation and BT-DMEM + UT-Test formulation groups at 50 μg / mL, respectively compared to the untreated group. Wound healing data showed significant wound closure and cell migration in all the tested groups in HaCaT cells compared to the untreated. Overall, the Consciousness Energy Healing (The Trivedi Effect®) Treated test formulation and DMEM have shown significant skin protective effects on various skin health parameters such as wrinkling, aging, skin whitening, and wound healing. Thus, the Biofield Treated test formulation would be suitable for the management of wounds and various skin-related disorders viz cellulitis, skin abscess, pimples, scabies, impetigo, syringoma, photosensitivity, urticaria, hives, warts, wrinkles, callus, acne, athlete’s foot,seborrheic dermatitis, chickenpox, eczema, psoriasis, erythematic, skin aging, contact dermatitis, etc.