Published: October 10, 2018 Volume: 3 Issue: 3 Pages: 124-132
DOI: 10.31586/Biochemistry.0303.04 ISSN: ISSN(Print): 2377-8091 ISSN(Online): 2377-8083
Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana
Citation: Kumar Trivedi, M., Branton, A., Trivedi, D., Nayak, G., Charan Mondal, S., & Jana, S. (2018). Effect of the Consciousness Energy Healing Treatment on DMEM for the Proliferation and Differentiation of Human Bone Osteosarcoma Cells to Improve Bone Health. Trends Journal Of Sciences Research, 3(3), 124-132. https://doi.org/10.31586/Biochemistry.0303.04
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The bone health is an important part of healthy-life and longevity in current situation due to huge toxins and contaminants in the environment and food chain. Considering the importance of bone health in the modern era, the present study was undertaken to investigate the effect of the Consciousness Energy Healing (The Trivedi Effect®) Treatment on Dulbecco's Modified Eagle Medium (DMEM) in which the human bone osteosarcoma cells - MG-63 (ATCC® CRL-1427™) was grown for the assessment of bone cell proliferation and differentiation in vitro. The study parameters were assessed using cell viability by MTT assay, alkaline phosphatase (ALP), and collagen synthesis on bone health using ELISA-based assay. The cell viability was significantly increased by 24% in the Biofield Energy Treated group supplemented with 10% charcoal-dextran with fetal bovine serum (CD-FBS) (G3) compared to the untreated cells group (G1). The level of ALP was significantly increased by 72% in the G3 group compared to the G1 group. Additionally, the level of collagen synthesis was significantly (p≤0.001) increased by 19% in the G3 group compared to the G1 group. The overall results demonstrated that the Biofield Energy Treated DMEM has the potential for bone mineralization and bone cells growth as evident via increased levels of collagen and ALP. Therefore, the Biofield Energy Healing (The Trivedi Effect®) Treatment could be useful as a bone health promoter for various bone-related disorders like low bone density, osteogenesis imperfecta, and osteoporosis, etc.
The cell viability was assessed using MTT assay of human bone osteosarcoma cells, which was grown in the Biofield Energy Treated DMEM culture. The MTT assay data found that Biofield Energy Healing showed a significantly increased the levels of proliferation in osteosarcoma cells compared to the untreated cells (G1) group. Besides, the level of ALP was significantly increased by 72% in the Biofield Energy Treated osteosarcoma cells group supplemented with 10% charcoal-dextran treated fetal bovine serum (CD-FBS) group (G3) compared to the G1 group. Besides, the level of collagen synthesis was also significantly increased by 19% in the G3 group compared to the G1 group. Overall, the Biofield Energy Treatment on DMEM culture medium enhanced bone mineralization and bone cells differentiation compared to the untreated cells group on human bone osteosarcoma cells. In conclusion, the Biofield Energy Healing might act as an effective bone health enhancer and it can be used as a complementary and alternative treatment for the prevention of various types of skeletal abnormality such as acromesomelic dysplasia, arthritis, bone erosion, bone metastasis, brachymetatarsia, osteoarthritis, caudal regression syndrome, chandler's disease, chondrodystrophy, opsismodysplasia, wrist osteoarthritis, zimmermann–laband syndrome, mastoiditis, ollier disease, rickets, oncogenic osteomalacia, handigodu syndrome, metachondromatosis, pacman dysplasia, osteochondrodysplasia, etc.