Differential Regulation of Immune Biomarkers by the Trivedi Effect®- Energy of Consciousness Healing Treatment Based Herbomineral Formulation in Male Sprague Dawley Rats

Journal: Biochemistry and Molecular Biology PDF  

Published: 11-Dec-17 Volume: 2 Issue: 6 Pages: 120-130

DOI: 10.11648/j.bmb.20170206.18 ISSN: 2575-5064 (Print) 2575-5048 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Ariadne Esmene Afaganis, Barbara Marie Bader, Brian A. Weekes, Daphne Luisa Dumas, Denise Marie Fiedler, Dennille Mellesia Smith, Desi Pano, Donna Felice Galla, Donna Maria Alija, Elaine Barbara Mullins, Elaine M. Scorza, Ellia O'Donnell, Fabio Massimo Paciucci, Frances Goodman Warlick, Haddon Norman Salt, Inthirani Arul, Jacqueline Y. Andrews, James Jay McLeran, James Stephen Burnett, Jean Caroline White, Mayank Gangwar, Snehasis Jana

Citation: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Ariadne Esmene Afaganis, Barbara Marie Bader, Brian A. Weekes, Daphne Luisa Dumas, Denise Marie Fiedler, Dennille Mellesia Smith, Desi Pano, Donna Felice Galla, Donna Maria Alija, Elaine Barbara Mullins, Elaine M. Scorza, Ellia O'Donnell, Fabio Massimo Paciucci, Frances Goodman Warlick, Haddon Norman Salt, Inthirani Arul, Jacqueline Y. Andrews, James Jay McLeran, James Stephen Burnett, Jean Caroline White, Mayank Gangwar, Snehasis Jana. Differential Regulation of Immune Biomarkers by the Trivedi Effect®- Energy of Consciousness Healing Treatment Based Herbomineral Formulation in Male Sprague Dawley Rats. Biochemistry and Molecular Biology. Vol. 2, No. 6, 2017, pp. 120-130. doi: 10.11648/j.bmb.20170206.18

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Abstract

A new proprietary herbomineral formulation was formulated, consisting of essential ingredients viz. herbal root extract of ashwagandha and minerals (zinc, magnesium, and selenium). The present study aimed to evaluate the impact of The Trivedi Effect®- Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) based herbomineral formulation in male Sprague Dawley (SD) rats for immune biomarkers modulation. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Treated sample, which received the Biofield Energy Healing Treatment remotely from twenty renowned Biofield Energy Healers. The immunomodulatory potential was studied in male SD rats using various immune biomarkers such as IgG and IgM, CD4+ and CD8+, hematology, lipid profile, hepatic enzymes, sex hormone, and antioxidant profile. The humoral immune response showed an increase IgG level by 6.10% in the Biofield Energy Treated test formulation (G4) group compared with the disease control (G2) group. Cellular immune response i.e. CD4+ and CD8+ counts were significantly improved by 51.64% and 52.09%, respectively in G4 compared with the G2 group. The lymphocytes level were increased by 38.87%, while TLC, neutrophils, eosinophils, and monocytes count were decreased by 7.38%, 19.35%, 21.14%, and 3.43%, respectively in G4 compared with the G2 group. Lipid analysis showed that triglycerides and VLDL levels were decreased by 6.02% and 5.97%, respectively while HDL level was significantly increased by 28.44% in G4 compared with the G2 group. Hepatic biomarkers viz. SGOT, CK-MB, total protein, total bilirubin, and globulin by 6.27%, 14.97%, 2.85%, 16.66%, and 5.90%, respectively in G4 compared with the G2 group. However, the testosterone level was significantly increased in G4 and untreated test formulation (G5) groups by 135.74% and 28.09%, respectively compared with the G2 group. Additionally, the antioxidant assay showed that the lipid peroxidation (LPO) level was decreased, while superoxide dismutase (SOD) and catalase levels were increased by 59.72% and 20.07%, respectively in the G4 compared with the G2 group. Overall, the study results suggested that the Biofield Energy Treated test formulation showed an improved cellular and humoral immune responses along with improved hematological and biochemical animal profile compared with the untreated test formulation. Consequently, it can be established that The Trivedi Effect®-Biofield Energy Healing has the significant capacity for immunomodulatory effect, which may also be useful in organ transplants, anti-aging, and stress management by improving overall health and quality of life.

Conclusion

The experimental results exhibited a significantly impact of The Trivedi Effect®- Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on test formulation with significant immunomodulatory action in male Sprague Dawley rats. The results of humoral immune biomarkers showed that the IgG level was increased by 6.10%, while IgM level remained unaltered in the Biofield Energy Treated test formulation (G4) group as compared with the disease control group (G2). In addition, the level of IgM was decreased by 13.03% in the untreated test formulation, thus the Biofield Energy Treated test formulation showed an improved results as compared with the untreated test formulation. Similarly, the cellular immune biomarkers (i.e. CD4+ and CD8+) levels were improved as compared with the disease control. The significant increase in the percentage of CD4+ and CD8+ levels were reported by 51.64% and 52.09%, in the Biofield Energy Treated test formulation (G4) compared with the G2 group. However, an improved cellular immune response was significant in the Biofield Energy Treated test formulation compared with the untreated test formulation. Besides, the blood differential test indicated a decreased level of TLC, neutrophils, lymphocytes, eosinophils, and monocytes by 7.38%, 19.35%, 21.14%, and 3.43%, respectively in the Biofield Energy Treated test formulation group (G4) compared with the disease control group (G2). However, the level of lymphocytes were increased by 38.87% in G4 compared with the G2 group. Biochemical analysis showed that the glucose concentration was altered (10.58%) and the level of LDL was increased by 31.84%, while VLDL and triglycerides were decreased by 5.97% and 6.02%, respectively in the Biofield Energy Treated test formulation (G4) group as compared with the disease control (G2) group. Although, the HDL level was significantly increased by 28.44% in G4 group compared with the G2 group. Further, the hepatic and cardiac biomarkers in serum sample were analyzed that showed decrease in the level of SGOT, CK-MB, TP, TB, and G by 6.27%, 14.97%, 2.85%, 16.66%, and 5.90%, respectively in the Biofield Energy Treated test formulation (G4) group compared with G2 group. The serum testosterone level was significantly increased by 135.74% in G4 group, while untreated test formulation showed only 28.09% increase in testosterone compared with the diseases control (G2) group. In addition, the antioxidant assay showed a significant decreased LPO level, while the level of SOD and catalase was significantly increased by 59.72% and 20.07%, respectively in G4 group compared with the G2 group.

Overall, the current experimental findings suggested that the Trivedi Effect®-Biofield Energy Healing Treatment done remotely by the twenty Biofield Energy Healers, which enhanced the herbomineral test formulation’s anti-inflammatory and immunomodulatory properties that can be used to improve the overall health. Thus, the Biofield Energy Treated test formulation may act as an effective anti-inflammatory and immunomodulatory product, and it can be used as a Complementary and Alternative Medicine (CAM) with a safe therapeutic index for various autoimmune disorders such as Lupus, Systemic Lupus Erythematosus, Fibromyalgia, Addison Disease, Hashimoto Thyroiditis, Celiac Disease (gluten-sensitive enteropathy), Multiple Sclerosis, Dermatomyositis, Graves’ Disease, Myasthenia Gravis, Pernicious Anemia, Aplastic Anemia, Scleroderma, Psoriasis, Rheumatoid Arthritis, Reactive Arthritis, Type 1 Diabetes, Sjogren Syndrome, Crohn’s Disease, Vasculitis, Vitiligo, Chronic Fatigue Syndrome and Alopecia Areata, as well as inflammatory disorders such as Irritable Bowel Syndrome (IBS), Asthma, Ulcerative Colitis, Alzheimer’s Disease, Parkinson’s Disease, Atherosclerosis, Dermatitis, Hepatitis, and Diverticulitis. Further, the Biofield Energy Healing Treated test formulation can also be used in the prevention of immune-mediated tissue damage in cases of organ transplants (for example heart transplants, kidney transplants and liver transplants), for anti-aging, stress prevention and management, and in the improvement of overall health and quality of life.