Cytoprotective Effect of Biofield Energy Treated Test Item against Tert-Butyl Hydroperoxide (T-BHP) – Induced Cell Damage in Hepg2 Cell-Line

Journal: Global Journal of Medical Research: B Pharma, Drug Discovery, Toxicology & Medicine PDF  Web

Published: 2018 Volume: XVIII Issue: VIII Pages: 7-15

ISSN: Online 2249-4618 Print: 0975-5888

Authors: Alice Branton & Snehasis Jana

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Abstract

Emerging data indicate that the mortality rate is rising due to liver disorders day-by-day in the developed countries. The present study was conducted to evaluate the potential of the Biofield Energy (The Trivedi Effect®) Treated test item (DMEM) in HepG2 cell-line. The test item was divided into two parts. One part of the test item received Consciousness Energy Healing Treatment by a renowned Biofield Energy Healer, Alice Branton and was labeled as the Biofield Energy Treated DMEM and the other part defined as untreated DMEM, where no Biofield Treatment was provided. Cell viability of the test items using MTT assay showed 113% and 129.9% viable cells in the untreated DMEM and Biofield Energy Treated DMEM groups, respectively suggested that the test items were nontoxic and safe in nature. The Biofield Energy Treated DMEM showed significant (p≤0.001) protection of cells by 15% against oxidative stress induced by t-BHP, while untreated DMEM group showed 0.4% protection.

Conclusion

The study results showed that the test items were safe and non-toxic based on MTT cell viability assay. The Biofield Energy Treated test item (DMEM) showed significant (p≤0.001) protection of cells by 15% from the oxidative damage induced by t-BHP, while untreated DMEM group showed 0.4% protection. The proinflammatory cytokine, IL-8 was significantly (p≤0.01) reduced by 32.15% in the Biofield Energy Treated DMEM group compared to the untreated DMEM group. Moreover, ALT enzyme activity was significantly (p≤0.01) reduced by 53.2% in the Biofield Energy Treated DMEM group compared to the untreated DMEM group. Cholesterol level was significantly (p≤0.001) reduced by 37.35% in the Biofield Energy Treated DMEM group compared to the untreated DMEM group. Further, Biofield Energy Treated DMEM group showed 43.13% increased the level of albumin compared to the untreated DMEM group. In conclusion, The Trivedi Effect®- Consciousness Energy Healing Treatment significantly protect hepatocytes cells oxidative stress and it can be used as a complementary and alternative treatment for the prevention of various types of hepatobiliary disorders viz. acute hepatitis A, B, C, D, and E, chronic viral hepatitis, portal hypertension in schistosomiasis, toxoplasmosis, hepatosplenic schistosomiasis, liver abscess, autoimmune hepatitis, primary biliary cholangitis (primary biliary cirrhosis), phlebitis of the portal vein, granulomatous hepatitis, cholestasis, necrosis, cirrhosis, etc. Further, it could be useful to improve cell-to-cell messaging, normal cell growth and differentiation, cell cycling and proliferation, neurotransmission, skin health, hormonal balance, immune and cardiovascular functions. Moreover, it can also be utilized in organ transplants (i.e., kidney, liver, and heart transplants), hormonal imbalance, aging, and various inflammatory and immune-related disease conditions like Alzheimer’s Disease (AD), Ulcerative Colitis (UC), Dermatitis, Asthma, Irritable Bowel Syndrome (IBS), Hashimoto Thyroiditis,