Biofield Energy Enriched Vitamin D3 versus Vitamin D3 in Preventing Fractures and Bone Loss Using MG-63 Cells

Journal: Orthopedic Research Online Journal PDF  

Published: July 17, 2018 Volume: 3 Issue: 5

DOI: 10.31031/OPROJ.2018.03.000572 ISSN: 2576-8875

Authors: Lorraine Marie Hachfeld, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana

Citation: Lorraine M H, Mahendra K T, Alice B, Dahryn T, Gopal N, et al . Biofield Energy Enriched Vitamin D3 versus Vitamin D3 in Preventing Fractures and Bone Loss Using MG-63 Cells.Ortho Res Online J. 3(5). OPROJ.000572.2018. DOI: 10.31031/OPROJ.2018.03.000572

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Abstract

Bone is a dynamic tissue, which continually adapting its structure during the development process. The aim of the study was to investigate the effect of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health parameters such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization using MG-63 cells. The test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Lorraine Marie Hachfeld and samples were defined as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability was more than 79% with a safe and nontoxic profile on MG-63 cell line. The level of ALP was increased by 400%, 84%, and 179.4% at 0.1, 1, and 10µg/mL, respectively in the UT-DMEM+BT-TI, while 314.3% at 10µg/mL in the BT-DMEM+UT-TI group as compared with the untreated test item and DMEM group. BT-DMEM+BT-TI group showed an increased ALP level by 128% and 60.3% at 1 and 10µg/mL, respectively. Collagen was significantly increased by 180% and 128.1% at 0.1 and 1µg/mL, respectively in the UT-DMEM+BT-TI group, while 84.4% and 69.7% at 1 and 10µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. Furthermore, BT-DMEM+BT-TI group showed a significant increased collagen level by 123.4% and 184.8% at 1 and 10 µg/mL, respectively as compared with the untreated test item and DMEM group. The percent of bone mineralization was significantly increased by 385.1% at 0.1µg/mL in the UT-DMEM+BT-TI group, while 53.2%, 30.2%, and 163.9% at 0.1, 1, and 10µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 283%, 65.1%, and 197.3% at 0.1, 1, and 10µg/mL, respectively as compared with the untreated group. The experimental data suggested that the Biofield Energy Treated vitamin D3 and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. It regulates the osteoblast function, improves the bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.

Conclusion

Biofield Energy Treated vitamin D3 and DMEM was studied for bone health parameters. MTT study for cell viability data showed that a significant improved cell viability with more than 79% among all the tested groups. The level of ALP was increased by 400%, 84%, and 179.4% at 0.1, 1, and 10µg/mL, respectively in the UT-DMEM+BT-TI, while 314.3% at 10µg/mL in the BT-DMEM+BT-TI group as compared with the untreated test item and DMEM group. BT-DMEM+BT-TI group showed an increased ALP level by 128% and 60.3% at 1 and 10µg/mL, respectively. The level of collagen was significantly increased by 180%, 128.1%, and 38.6% at 0.1, 1, and 10µg/mL, respectively in the UT-DMEM+BT-TI, while 84.4% and 69.7% at 1 and 10µg/mL, respectively in the BT-DMEM+UT-TI group. In addition, collagen level was increased by 44.4%, 123.4%, and 184.8% at 0.1, 1, and 10µg/mL, respectively in BT-DMEM+BT-TI group as compared with the untreated test item and DMEM group. Similarly, the bone mineralization percent was significantly increased by 385.1%, 28.7%, and 19.7% at 0.1, 1, and 10µg/mL, respectively in the UT-DMEM+BT-TI group, while 53.2%, 30.2%, and 163.9% at 0.1, 1, and 10µg/mL, respectively in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 283%, 65.1%, and 197.3% at 0.1, 1, and 10µg/mL, respectively as compared with the untreated group. Thus, experimental results demonstrated that Biofield Energy Healing Treatment can be utilized in managing various bone health parameters viz. collagen, bone mineralization, and ALP to combat the bone disorders. Thus, The Trivedi Effect®-Consciousness Energy Healing based vitamin D3 showed a significant improved bone health, which supports its use as the best nutritional supplement to treat bone related disorders such as rickets, deformed bones, osteomalacia, osteoma, osteoporosis, Paget’s disease, aging, bone and/or joint pain, increased tendency of fractures, hormonal imbalance, stress, and other associated bone diseases. Besides, this approach can also supports its use against many various immune related disease conditions and worked as anti-arthritic, anti-aging, anti-apoptotic, anti-osteoporosis, anti-inflammatory, anti-stress, anti-cancer, wound healing, and anti-fibrotic roles. It would improve the cell-to-cell communication, cell differentiation, cell cycling and proliferation, normal cell growth, neurotransmission, skin health, and also in vital cardiovascular functions. Besides, it can also be utilized against Dermatitis, Multiple Sclerosis, Pernicious Anemia, Asthma, Irritable Bowel Syndrome, Diabetes, Diverticulitis, Hashimoto Thyroiditis, Myasthenia Gravis, Sjogren Syndrome, Aplastic Anemia, Hepatitis, Parkinson’s disease, Atherosclerosis, and many more.