Bacterial Identification Using 16S rDNA Gene Sequencing and Antibiogram Analysis on Biofield Treated Pseudomonas fluorescens

Journal: Clinical & Medical Biochemistry: Open Access PDF  

Published: 4 Sep 15 Volume: 1 Issue: 1

DOI: 10.4172/2471-2663.1000101 ISSN: 2471-2663

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar and Snehasis Jana*

Citation: Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, et al. (2015) Bacterial Identification Using 16S rDNA Gene Sequencing and Antibiogram Analysis on Biofield Treated Pseudomonas fluorescens. Clin Med Biochemistry Open Access 1:101. doi:10.4172/cmbo.1000101

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Abstract

Biofield therapies have been reported to improve the quality of life as compared to other energy medicine. The aim of the study was to evaluate the impact of Mr. Trivedi’s biofield energy treatment on Pseudomonas fluorescens (P. fluorescens) for antimicrobial sensitivity, minimum inhibitory concentration (MIC), biochemical reactions, and biotype number. P. fluorescens cells were procured from MicroBioLogics Inc., USA in sealed packs bearing the American Type Culture Collection (ATCC 49838) number and divided in control and treated group. The effect was evaluated on day 10, and 159 after biofield treatment in lyophilized state. Further study was performed on day 5, 10, and 15 after retreatment on day 159 in revived state as per study design. All experimental parameters were studied using automated MicroScan Walk-Away® system. The 16S rDNA sequencing was carried out to correlate the phylogenetic relationship of P. fluorescens with other bacterial species after treatment. The results showed improved sensitivities and decreased MIC value of aztreonam, cefepime, moxifloxacin, and tetracycline in revived and lyophilized treated sample with respect to the control. Arginine, cetrimide, kanamycin, and glucose showed altered biochemical reactions after biofield treatment with respect to control. Biotype numbers were altered along with species in lyophilized as well as in revived group. Based on nucleotides homology and phylogenetic analysis using 16S rDNA gene sequencing, treated sample was detected to be Pseudomonas entomophila (GenBank Accession Number: AY907566) with 96% identity of gene sequencing data, which was nearest homolog species to P. fluorescens (Accession No. EF672049). These findings suggest that Mr. Trivedi’s unique biofield treatment has the capability to alter changes in pathogenic P. fluorescens even in the lyophilized storage condition and can be used to modify the sensitivity of microbes against antimicrobials.

Conclusion

Improved antimicrobial sensitivity and decreased MIC value of aztreonam, cefepime, moxifloxacin, and tetracycline in biofield treated Pseudomonas fluorescens showed significant impact of Mr. Trivedi’s biofield energy treatment. This approach can be used as a treatment approach in complementary and alternate medicine. Significant alteration in antimicrobial data was well supported with altered biochemical reactions along with biotype number. Molecular approach using standard 16S rDNA analysis showed that biofield treatment has significant impact on P. fluorescens, and sample identified as Pseudomonas entomophila with 96% identity using gene sequencing data. However, the closest homolog species was detected to be Pseudomonas fluorescens. Based on these results, it seems that Mr. Trivedi’s biofield energy treatment could be used as better alternate of existing drug therapy in future.