Antioxidative Potential of Biofield Energy Treated DMEM in Pulmonary Disease Using Lung Adenocarcinoma Cell Line (A549)

Journal: Applied Clinical Pharmacology and Toxicology  Web

Published: 24 April, 2019 Volume: 2019 Issue: 1 Pages: 1-6

DOI: 10.29011/ACPT-119.100019 ISSN: 2577-0225

Authors: Dahryn Trivedi, Snehasis Jana

Citation: Trivedi D and Jana S (2019) Antioxidative Potential of Biofield Energy Treated DMEM in Pulmonary Disease Using Lung Adenocarcinoma Cell Line (A549). Appl Clin Pharmacol Toxicol: ACPT-119. DOI : 10.29011/ACPT-119.100019

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Abstract

The present in vitro investigation was aimed to explore the effect of Consciousness Energy Healing Treated DMEM medium for oxidative potential using oxidative biomarkers viz. Superoxide Dismutase (SOD) enzyme activity and protection against oxidative stress measured in A549 cells. The test item, DMEM was divided into two parts. First part of the test item received Consciousness Energy Healing Treatment by a renowned Biofield Energy Healer, Dahryn Trivedi and was labeled as the Biofield Energy Treated DMEM group, while the second part did not receive any sort of Treatment and defined as the Untreated DMEM (control) group. The cell viability of the test sample using MTT assay showed an increase cell viability by 136.4% in the Biofield Energy Treated DMEM group, suggesting a safe and nontoxic nature of the test items. Protection against oxidative stress was significantly increased by 23.80% in the Biofield Energy Treated DMEM group compared with the untreated DMEM group. Besides, SOD enzyme activity was significantly increased by 40.40% in the Biofield Energy Treated DMEM group compared with the untreated DMEM group. Thus, the overall data suggest that the Biofield Energy Healing Treatment showed a significant improvement of SOD enzyme level along with an improved protection against oxidative damage, which can be used in various human lungs disorders such as asthma, Chronic Obstructive Pulmonary Disease (COPD), lung malignancies and parenchymal lung diseases like idiopathic pulmonary fibrosis and lung granulomatous diseases.

Conclusion

The cell viability using MTT assay showed 136.40% viable cells in the Biofield Energy Treated DMEM group indicating a safe and non-toxic profile of the test item. Moreover, the cellular protection against oxidative stress induced by t-BHP was significantly increased by 23.80% in the Biofield Energy Treated DMEM group compared with the untreated DMEM group. Besides, SOD enzyme activity was significantly increased by 40.40% in the Biofield Energy Treated DMEM group compared with the untreated DMEM group. Thus, the experimental result suggests that the Biofield Energy Treated (The Trivedi Effect®) DMEM were found to have a significant impact to protect from oxidative damage in adenocarcinoma cells (A549). It can also be useful for the management of various human lung disorders such as Bronchiectasis, Asbestosis, Asthma, Bronchitis, Chronic Cough, Chronic Obstructive Pulmonary Disease (COPD), Common Cold, Cystic Fibrosis, Hantavirus, Idiopathic Pulmonary Fibrosis, Influenza, Lung Cancer, Pertussis, Pandemic Flu, Pleurisy, Pneumonia, Pulmonary Embolism, Respiratory Syncytial Virus (RSV), Sarcoidosis, Sleep Apnea, Sudden Infant Death Syndrome (SIDS), Tuberculosis, and Work-Related Asthma. Besides, it may also control the immune related disease conditions such as Hashimoto Thyroiditis, Aplastic Anemia, Hepatitis, Diverticulitis, Pernicious Anemia, Sjogren Syndrome, Myasthenia Gravis, Parkinson’s Disease, Graves’ Disease, Dermatomyositis, Multiple Sclerosis, Ulcerative Colitis, Alzheimer’s Disease, Dermatitis, Irritable Bowel Syndrome, Diabetes, Atherosclerosis, Systemic Lupus Erythematosus, stress, etc. with a safe therapeutic index to improve overall health, and the Quality of Life.