Antibiogram Typing and Biochemical Characterization of Klebsiella pneumoniae after Biofield Treatment

Journal: Journal of Tropical Diseases PDF  

Published: 20 Aug 15 Volume: 3 Issue: 4

DOI: 10.4172/2329-891X.1000173 ISSN: 2329-891X

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Harish Shettigar, Mayank Gangwar and Snehasis Jana*

Citation: Trivedi MK, Branton A, Trivedi D, Shettigar H, Gangwar M, et al. (2015) Antibiogram Typing and Biochemical Characterization of Klebsiella Pneumonia after Biofield Treatment. J Trop Dis 3: 173. doi:10.4173/2329891X.1000173

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Abstract

Klebsiella pneumoniae (K. pneumoniae) is a common nosocomial pathogen causing respiratory tract (pneumoniae) and blood stream infections. Multidrug-resistant (MDR) isolates of K. pneumoniae infections are difficult to treat in patients in health care settings. Aim of the present study was to determine the impact of Mr. Trivedi’s biofield treatment on four MDR clinical lab isolates (LS) of K. pneumoniae (LS 2, LS 6, LS 7, and LS 14). Samples were divided into two groups i.e. control and biofield treated. Control and treated groups were analyzed for antimicrobial susceptibility pattern, minimum inhibitory concentration (MIC), biochemical study and biotype number using MicroScan Walk-Away® system. The analysis was done on day 10 after biofield treatment as compared with control group. Antimicrobial sensitivity assay showed that there was 46.42% alteration in sensitivity of tested antimicrobials in treated group of MDR K. pneumonia isolates. MIC results showed an alteration in 30% of tested antimicrobials out of thirty after biofield treatment in clinical isolates of K. pneumoniae. An increase in antimicrobial sensitivity and decrease in MIC value was reported (in LS 6) in case of piperacillin/tazobactam and piperacillin. Biochemical study showed a 15.15% change in biochemical reactions as compared to control. A significant change in biotype numbers were reported in all four clinical isolates of MDR K. pneumoniae after biofield treatment as compared to control group. On the basis of changed biotype number after biofield treatment, new organism was identified as Enterobacter aerogenes in LS 2 and LS 14. These results suggest that biofield treatment has a significant effect on altering the antimicrobial sensitivity, MIC values, biochemical reactions and biotype number of multidrug-resistant isolates of K. pneumoniae.

Conclusion

Overall data conclude that there has a significant impact of biofield treatment on antimicrobial susceptibility pattern, MIC values, biochemical reactions, and biotype number in all the four clinical MDR lab isolates of K. pneumoniae. Based on the study outcome, biofield treatment could be applied to alter the sensitivity pattern of antimicrobials, against multi-drug resistance isolates of K. pneumoniae.