Antibiogram and Genotypic Analysis using 16S rDNA after Biofield Treatment on Morganella morganii

Journal: Advanced Techniques in Biology & Medicine PDF  

Published: 17 Sep 15 Volume: 3 Issue: 3

DOI: 10.4172/2379-1764.1000137 ISSN: 2379-1764

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar and Snehasis Jana *

Citation: Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, et al. (2015) Antibiogram and Genotypic Analysis using 16S rDNA after Biofield Treatment on Morganella morganii. Adv Tech Biol Med 3: 137. doi: 10.4172/2379-1764.1000137

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Abstract

Morganella morganii (M. morganii) is one of the important nosocomial pathogen associated with the urinary tract infections and bacteremia. The aim of this study was to evaluate the effect of Mr. Trivedi’s biofield energy treatment on M. morganii in the lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, biotype number and genotype. M. morganii cells were procured from MicroBioLogics Inc., USA in sealed packs bearing the American Type Culture Collection (ATCC 25829) number and stored according to the recommended storage protocols until needed for experiments. M. morganii strain was divided into two groups, Group (Gr.) I: control and Gr. II: treated. Gr. II was further subdivided into two groups, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, while Gr. IIB was stored and analyzed on day 142 (Study I). After retreatment on day 142, the sample (Study II) was divided into three separate tubes. First, second and third tube was further analyzed on day 5, 10 and 15 respectively. All experimental parameters were studied using the automated MicroScan Walk-Away® system. The 16S rDNA sequencing of lyophilized treated sample was carried out to correlate the phylogenetic relationship of M. morganii with other bacterial species. Antimicrobial susceptibility results showed 32.14% alterations, while minimum inhibitory concentration results showed 18.75% alterations of the tested antimicrobials. Biochemical study also showed altered positive reactions in nitrofurantoin and indole with respect to control. Biotype study showed alteration in Gr. IIB, study II, on day 15 (4005 1446) as compared to the control (4004 1446). 16S rDNA sequencing analysis showed similar results with the identified microbe as M. morganii (GenBank accession number: AB210972) having 80% identity of the gene sequencing data. Total 1507 base nucleotide of 16S rDNA gene sequences were analyzed by multiple alignments, while nearest homolog genus-species of M. morganii was found as Providencia rettgeri (accession number: AM040492). These results suggested that biofield treatment has a significant impact on M. morganii in lyophilized as well as revived state

Conclusion

In conclusion, Mr. Trivedi’s biofield treatment on M. morganii showed the altered antimicrobial sensitivity of 32.14% tested antimicrobials. The MIC values of 18.75% tested antimicrobials were altered after biofield energy treatment in M. morganii. Characteristics biochemical test of M. morganii such as indole and nitrofurantoin reactions were altered followed by a change in the biotype number (4005 1446, Gr. IIB, study II) as compared to control (4004 1446). Thus, Mr. Trivedi’s unique biofield energy treatment could be applied to alter the antimicrobials resistance pattern. Molecular based 16S rDNA analysis showed that the identifiable sample in this experiment was detected as M. morganii (GenBank Accession Number: AB210972) with 80% identity of the gene sequencing data after biofield treatment. Based on the phylogenetic tree and 16S rDNA sequencing, the nearest homolog species was found as Providencia rettgeri (Accession No. AM040492). Based on these results, it seems that Mr. Trivedi’s biofield energy treatment could be used as an alternate of the existing drug therapy in future.