An Impact of the Trivedi Effect® – Biofield Energy Healing on Herbomineral Formulation for Immunomodulation of Pro-inflammatory Cytokines in Biofield Treated Mouse Splenocytes

Journal: Advances in Biochemistry PDF  

Published: 2016/12/08 Volume: 4 Issue: 6 Pages: 74-83

DOI: 10.11648/j.ab.20160406.12 ISSN: 2339-0870 (Print) 2329-0862 (Online)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Alan Joseph Balmer, Dimitrius Anagnos, Janice Patricia Kinney, Joni Marie Holling, Joy Angevin Balmer, Lauree Ann Duprey-Reed, Vaibhav Rajan Parulkar, Mayank Gangwar, Snehasis Jana

Citation: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Alan Joseph Balmer, Dimitrius Anagnos, Janice Patricia Kinney, Joni Marie Holling, Joy Angevin Balmer, Lauree Ann Duprey-Reed, Vaibhav Rajan Parulkar, Mayank Gangwar, Snehasis Jana. An Impact of the Trivedi Effect® - Biofield Energy Healing on Herbomineral Formulation for Immunomodulation of Pro-inflammatory Cytokines in Biofield Treated Mouse Splenocytes. Advances in Biochemistry. Vol. 4, No. 6, 2016, pp. 74-83. doi: 10.11648/j.ab.20160406.12

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Abstract

Herbomineral formulations have increased in recognition and popularity due to their high safety and better therapeutic action. A new proprietary herbomineral formulation was formulated with a mixture of the herbal root extract of ashwagandha and three minerals viz. zinc, magnesium, and selenium. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the test formulation when applied to splenocyte cells isolated from the Biofield Treated mice. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment. The other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely by seven renowned Biofield Energy Healers. A wide concentration range (0.00001053 to 10.53 µg/mL) of the test formulation was used to determine non-cytotoxic concentrations using MTT assay. Further, the expression of pro-inflammatory cytokines (TNF-α, MIP-1α, and IL-1β) was determined by ELISA method. The test formulation was evaluated and found to be safe up to 1.053 µg/mL with a percentage cell viability range of 73% to 97% using MTT assay. The Biofield Treated formulation improved the cell viability up to 6.61% compared with the untreated test formulation. TNF-α expression was significantly inhibited by 16.72% at 0.1053 µg/mL compared with the untreated test formulation, however expression was significantly altered by 53.67% and 25.62% at 0.01053 and 1.053 µg/mL, respectively compared to the untreated test formulation. TNF-α expression was also suppressed in the Biofield Treated test formulation at 0.001053 and 0.1053 µg/mL by 4.0% and 8.56%, respectively as compared with the vehicle control. MIP-1α suppression was reported in the Biofield Treated test formulation at 0.00001053 to 1.053 µg/mL by 8.43%, 22.02%, 21.92%, 20.54%, 5.40%, and 19.82%, respectively compared with the vehicle control. However, the Biofield Treated formulation further exhibited substantial suppression of MIP-1α at 0.0001053, 0.001053, 0.01053, and 0.1053 µg/mL by 13.50%, 7.38%, 36.83% (p≤0.001), and 2.53%, respectively compared with the untreated test formulation. In addition, significant inhibition of IL-1β secretion was reported in the Biofield Treated formulation at 0.0001053, 0.001053, 0.01053, and 0.01053 µg/mL by 32.40%, 14.99%, 60.42%, and 15.15%, respectively compared with the untreated test formulation. The Biofield Energy Healing Treatment significantly potentiated the immunosuppressive effect of the test formulation in Biofield Treated mouse splenocytes, which can be used for autoimmune and inflammatory diseases, stress management and anti-aging by improving overall health.

Conclusion

Based on the current findings of this study, the splenocyte cells isolated from the Biofield Energy Treated mice showed significant immunosuppressive effect on the tested cytokines (TNF-α, MIP-1α, and IL-1β) after administration of the Biofield Treated test formulation as compared with the untreated test formulation. MTT assay in the Biofield Treated splenocyte cells suggest that the Biofield Energy Treated formulation increased the cell viability by 3.17%, 5.07%, 2.77%, and 6.61% at 0.0001053, 0.01053, 0.1053, and 1.053 µg/mL, respectively as compared with the untreated test formulation. Further, the levels of cytokines were significantly suppressed in the Biofield Energy Treated test formulation group. TNF-α level was significantly inhibited by 16.72% at 0.1053 µg/mL in the Biofield Treated test formulation as compared with the untreated test formulation. However, the Biofield Energy Treated formulation group also showed low levels of TNF-α expression by 4.0% and 8.56% at 0.001053 and 0.1053 µg/mL, respectively as compared with the vehicle control group. In the case of MIP-1α, the Biofield Energy Treated test formulation showed significant suppression by 13.50%, 7.38%, 36.83% (p≤0.001), and 2.53% at 0.0001053, 0.001053, 0.01053, and 0.1053 µg/mL, respectively as compared with the untreated test formulation. Besides, the levels of MIP-1α were also downregulated in all the tested concentrations of the test formulation by 8.43%, 22.02%, 21.92%, 20.54%, 5.40%, and 19.82% at 0.00001053, 0.0001053, 0.001053, 0.01053, 0.1053 and 1.053 µg/mL, respectively as compared with the vehicle control group. Suppression of IL-1β expression was also reported in the Biofield Energy Treated test formulation group by 32.40%, 14.99%, 60.42% (p≤0.05), and 15.15% at 0.0001053, 0.001053, 0.01053, and 0.01053 µg/mL, respectively as compared with the untreated formulation.

On the basis of the experimental results of the various tested cytokines and their expression, significant immunosuppressive activity was reported in the new herbomineral formulation after treatment with The Trivedi Effect® - Biofield Energy Healing (TEBEH) by the group of seven renowned Biofield Energy Healers. The Biofield Energy Treated test formulation can be used as an effective Complementary and Alternative Medicine (CAM) approach to prevent and treat immune-mediated diseases such as Irritable Bowel Syndrome, Rheumatoid arthritis, Ulcerative colitis and Crohn's disease, Stress, Asthma, and many more, with a safe therapeutic index. Biofield Energy Healing Treatments can also be utilized in organ transplants (for example kidney transplants, liver transplants and heart transplants), various autoimmune disorders such as Lupus, Addison Disease, Celiac Disease (gluten-sensitive enteropathy), Dermatomyositis, Graves’ Disease, Hashimoto Thyroiditis, Multiple Sclerosis (MS), Myasthenia Gravis, Pernicious Anemia, Aplastic Anemia, Sjogren Syndrome, Systemic Lupus Erythematosus, Alopecia Areata, Fibromyalgia, Vitiligo, Psoriasis, Scleroderma, Chronic Fatigue Syndrome, Vasculitis, and Type 1 Diabetes. Biofield Energy Healing can also be utilized for the anti-inflammatory disorders, stress prevention and management, anti-aging, and for the improvement of overall health and quality of life.