A Comparative Analysis of Vitamin D3 and Biofield Energy Treated Vitamin D3 in Bone Health Using MG-63 Cell Line

Journal: Orthoplastic Surgery & Orthopedic Care International Journal PDF  

Published: August 1, 2018 Volume: 1 Issue: 5 Pages: 1-7

DOI: 10.31031/OOIJ.2018.01.000525 ISSN: 2578-0069

Authors: Lauree Ann Duprey-Reed, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar and Snehasis Jana

Citation: Lauree A D R, Mahendra K T, Alice B, Dahryn T, Gopal N, et al. A Comparative Analysis of Vitamin D3 and Biofield Energy Treated Vitamin D3 in Bone Health Using MG-63 Cell Line. Ortho Surg Ortho Care Int J . 1(5). OOIJ.000525.2018. DOI: 10.31031/OOIJ.2018.01.000525

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Abstract

Poor bone health is related with various bone disease that predisposes to fractures, while sufficient supplementation with calcium and vitamin D suggested prevention of bone diseases. The present study aimed to find the effect of Consciousness Energy Healing based vitamin D3 and DMEM medium on in vitro bone health parameters using MG-63 cells such as alkaline phosphates enzyme (ALP) activity, collagen levels, and bone mineralization. The test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Lauree Ann Duprey-Reed and samples were defined as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability range 76% to 139% with safe and nontoxic profile of test samples on MG-63 cell line. ALP was significantly increased by 171.8% and 189.7% at 10µg/mL in the UT-DMEM+BT-TI and BT-DMEM+BT-TI groups, respectively as compared with the untreated group. In addition, 100% and 33.3% increased ALP at 0.1 and 10µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. The level of collagen was significantly increased at 10µg/mL by 145.8% in the UT-DMEM+BT-TI group, while 65.8% and 145.8% at 1 and 10µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased collagen level by 86.3% and 136.1% at 1 and 10µg/mL, respectively as compared with the untreated test item and DMEM group. The percent of bone mineralization was significantly increased by 150%, 21%, and 73.2% at 0.1, 10, and 100µg/mL, respectively in the UTDMEM+BT-TI group, while 258.3% and 126.8% at 0.1 and 100µg/mL, respectively in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 33.3%, 32.7%, and 187.4% at 0.1, 10, and 100µg/ mL, respectively as compared with the untreated group. Biofield Energy Treatment might be vital in promotion and maintenance of strong and healthy bones and quality of life by assisting them in maintaining optimal vitamin D levels. It regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer, and many autoimmune disorders.

Conclusion

Biofield Energy Treated vitamin D3 demonstrated significant improved bone health parameters in MG-63 cells. MTT assay data showed that a significant improved cell viability with more than76% among various test groups. Bone health parameters such as the level of ALP were increased by 171.8% at 10µg/mL in the UT-DMEM+BT-TI, while 100% and 33.3% at 0.1 and 10µg/mL, re-spectively in the BT-DMEM+UT-TI group as compared with the un-treated test item and DMEM group. In addition, BT-DMEM+BT-TI group showed an increased ALP level by 50% and 189.7% at 0.1 and 10µg/mL, respectively. The level of collagen was significantly increased by 35.6% and 145.8% at 1 and 10µg/mL, respectively in the UT-DMEM+BT-TI, while 65.8% and 145.8% at 1 and 10µg/mL, respectively in the BT-DMEM+UT-TI group. The level of collagen was increased by 86.3%, 136.1%, and 15.1% at 1, 10, and 50µg/mL, respectively in BT-DMEM+BT-TI group as compared with the untreated test item and DMEM group. Similarly, the bone miner-alization percent was significantly increased by150%, 21%, and 73.2% at 0.1, 10, and 100µg/mL, respectively in the UT-DMEM+BT-TI group, while 258.3% and 126.8% at 0.1 and 100µg/mL, respec-tively in the BT-DMEM+UT-TI group as compared with the untreat-ed group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 33.3%, 32.7%, and 187.4% at 0.1, 10, and 100µg/mL, respectively as compared with the untreated group. Thus, experimental results demonstrated that The Trivedi Effect® treated test samples can be utilized in managing various bone health parameters viz. collagen, bone mineralization, and ALP to combat the bone disorders. Thus, The Trivedi Effect® based vita-min D3 showed a significant improved bone health, which supports its use as the best nutritional supplement to treat bone related dis-orders such as rickets, deformed bones, osteomalacia, osteoma, os-teoporosis, Paget’s disease, aging, bone and/or joint pain, increased tendency of fractures, hormonal imbalance, stress, and other asso-ciated many more bone diseases.